Vector Labs Ab03389-23.0 Anti-OPGbp [AT], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: AT
Synonyms: Osteoprotegerin binding protein; Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin
Amount: 200 μg
Application Codes Clone: Inhibition; ELISA; SPR
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody recognizes Human OPGbp (residues 143-317). OPGbp acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. It inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The binding affinity of the Fab and IgG1 fragments of the antibody to OPGbp was measured by surface plasmon resonance (Kd≈ 140 nM and 0.33-0.43 nM respectively). The interaction of human OPGbp and its receptor ODAR was measured in a fluorescence energy transfer (FRET) assay. The Fab fragment of the antibody inhibited OPGbp binding to soluble ODAR-Fc fusion with an IC50 of 550 nM. Bone Marrow Assay using the Fab fragment of the antibody gave a 50% decrease (IC50) at 57.8 nM. In RAW Cell Assay the Fab fragment of the antibody showed an IC50 of 15 μg/ml, 300 nM. In RAW Cell Assay, the 3 full length versions of the antibody “AT”405, “AT”406 and “AT”407 showed IC50 of 20.1 nM, 60.3 nM & 21.4 nM, respectively. “AT”405 or “AT”407 were tested in murine bone marrow assay, showing IC50 of 2.15 ug/ml (14.4 nM), and 1.85 ug/ml (12.5 nM), respectively. “AT”406 was tested in the bone marrow coculture assay and inhibited TRAP formation with an IC50 of 2.35 μg/ml (14.4 nM) (US20140147444).

Product Class:	Purified
Clone ID:	AT
Synonyms:	Osteoprotegerin binding protein; Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin
Amount:	200 μg
Application Codes Clone:	Inhibition; ELISA; SPR
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody recognizes Human OPGbp (residues 143-317). OPGbp acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. It inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro.
Application Notes:	The specificity of the original format of the antibody was confirmed by ELISA analysis. The binding affinity of the Fab and IgG1 fragments of the antibody to OPGbp was measured by surface plasmon resonance (Kd≈ 140 nM and 0.33-0.43 nM respectively). The interaction of human OPGbp and its receptor ODAR was measured in a fluorescence energy transfer (FRET) assay. The Fab fragment of the antibody inhibited OPGbp binding to soluble ODAR-Fc fusion with an IC50 of 550 nM. Bone Marrow Assay using the Fab fragment of the antibody gave a 50% decrease (IC50) at 57.8 nM. In RAW Cell Assay the Fab fragment of the antibody showed an IC50 of 15 μg/ml, 300 nM. In RAW Cell Assay, the 3 full length versions of the antibody “AT”405, “AT”406 and “AT”407 showed IC50 of 20.1 nM, 60.3 nM & 21.4 nM, respectively. “AT”405 or “AT”407 were tested in murine bone marrow assay, showing IC50 of 2.15 ug/ml (14.4 nM), and 1.85 ug/ml (12.5 nM), respectively. “AT”406 was tested in the bone marrow coculture assay and inhibited TRAP formation with an IC50 of 2.35 μg/ml (14.4 nM) (US20140147444).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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