Mfg Part Number
Ab03407-23.0
Vector Labs Ab03407-23.0 Anti-Annexin XI [A5A3], Rabbit IgG, lambda (200 μg)
Quick Overview
The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.
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| Product Class: | Purified |
|---|---|
| Clone ID: | A5A3 |
| Synonyms: | Annexin A11; 56 kDa autoantigen; Annexin-11; Calcyclin-associated annexin 50; CAP-50 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody is specific for human annexin XI. Annexin XI binds specifically to calcyclin in a calcium-dependent manner and it is required for midbody formation and completion of the terminal phase of cytokinesis. |
| Application Notes: | The binding characterization of this antibody was done using ELISA. This antibody is also capable of recognizing human annexin XI in a western blot assay (Hof et al., 2008; PMID: 18062792). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |