Mfg Part Number
Ab03413-23.0
Vector Labs Ab03413-23.0 Anti-envelope protein [ZIKVEF2L01], Rabbit IgG, lambda (200 μg)
Quick Overview
The original antibody was generated by immunizing chicken with ZIKV envelope protein followed by antibody library construction and phage display-based selection.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | ZIKVEF2L01 |
| Synonyms: | ZIKV envelope protein; E protein |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; FC; in vitro inhibition |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody binds to the E protein of Zika virus. The envelope protein forms a dimer on the smooth surface of the mature virus, and participates in receptor binding and fusion to the host. |
| Application Notes: | The specificity of the scFv format of the antibody was confirmed by ELISA analysis. The antibody detected the recombinant E protein by western blot analysis. The scFv format recognized the E protein in a transfected Vero cell by flow cytometry. Competitive inhibition assay was employed to calculate the percentage of inhibition and the dissociation constant of the antibody against the E protein (inhibition of 50% = 0.55 ug/mL and Kd= 2.04 *10^-8 M). Therefore, the antibody has diagnostic or therapeutic potential for ZIKV (PMID: 31940993). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |