Vector Labs Ab03415-10.0 Anti-Claudin-4 [KM3900], Human IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: KM3900
Synonyms: CLDN4; Claudin 4; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein; FERM BP-10751
Amount: 200 μg
Application Codes Clone: FC; IP; Neutralizing; Therapeutic
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody specifically recognizes the conformational structure and binds to extracellular loop 2 of CLDN4. The antibody does not react with denatured CLDN4 protein. The antibody does not cross react with CLDN3, 5, 6, and 9. Claudin-4 is a tetraspanin transmembrane protein of tight junction structure and is highly expressed in pancreatic and ovarian cancers.
Application Notes: Cell lysates were prepared from pancreatic cancer cells (Capan-2 and HPAF-II) and CLDN/CHO cells, and subjected to immunoprecipitation analysis. The antibody was shown to specifically precipitate CLDN4. This antibody detected CLDN4 expressed on CHO cells by flow cytometry. Furthermore, binding of the antibody was detected on CLDN4-expressing pancreatic and ovarian cancer cells, but not on negative cells. Moreover, the chimeric IgG1 (KM3934) version of the antibody was made and its antitumor efficacy was evaluated. KM3934 induced dose-dependent antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity in vitro, and significantly inhibited tumor growth in MCAS or CFPAC-1 xenograft SCID mice in vivo (Suzuki et al., 2009; PMID:19555390). The antibody strongly bound to the CLDN4 expressed in breast cancer cells, colorectal cancer cells and prostate cancer cells. The antibody inhibited the cell movement ability of CLDN4 expression cells. The antibody showed neutralizing activity for CLDN4 (US8076458).

Product Class:	Purified
Clone ID:	KM3900
Synonyms:	CLDN4; Claudin 4; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein; FERM BP-10751
Amount:	200 μg
Application Codes Clone:	FC; IP; Neutralizing; Therapeutic
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody specifically recognizes the conformational structure and binds to extracellular loop 2 of CLDN4. The antibody does not react with denatured CLDN4 protein. The antibody does not cross react with CLDN3, 5, 6, and 9. Claudin-4 is a tetraspanin transmembrane protein of tight junction structure and is highly expressed in pancreatic and ovarian cancers.
Application Notes:	Cell lysates were prepared from pancreatic cancer cells (Capan-2 and HPAF-II) and CLDN/CHO cells, and subjected to immunoprecipitation analysis. The antibody was shown to specifically precipitate CLDN4. This antibody detected CLDN4 expressed on CHO cells by flow cytometry. Furthermore, binding of the antibody was detected on CLDN4-expressing pancreatic and ovarian cancer cells, but not on negative cells. Moreover, the chimeric IgG1 (KM3934) version of the antibody was made and its antitumor efficacy was evaluated. KM3934 induced dose-dependent antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity in vitro, and significantly inhibited tumor growth in MCAS or CFPAC-1 xenograft SCID mice in vivo (Suzuki et al., 2009; PMID:19555390). The antibody strongly bound to the CLDN4 expressed in breast cancer cells, colorectal cancer cells and prostate cancer cells. The antibody inhibited the cell movement ability of CLDN4 expression cells. The antibody showed neutralizing activity for CLDN4 (US8076458).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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