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Mfg Part Number

Ab03419-10.0

Vector Labs Ab03419-10.0 Anti-Desmoglein-3 [F779], Human IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated from the peripheral blood of four patients with active untreated pemphigus vulgaris (PV) using heterohybridoma techniques.

$517.40

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Description

Product Class: Purified
Clone ID: F779
Synonyms: DSG3; PVA; CDHF6; Desmoglein 3; 130 kDa pemphigus vulgaris antigen; Cadherin family member 6
Amount: 200 μg
Application Codes Clone: ELISA; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds human desmoglein-3 between amino acids 1-161 and does not cross react with desmoglein-1. It is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Pemphigus vulgaris (PV) is a potentially lethal skin disease in which epidermal blisters occur as the result of the loss of cell-cell adhesion caused by the action of autoantibodies against desmoglein 3. It is characterized by suprabasal blistering of skin and mucous membranes.
Application Notes: This antibody can be used for the determination of desmoglein-3 using ELISA. The binding affinity of IgG1 version of this antibody for human desmoglein-3 was determined using surface plasmon resonance and was found to be Kd = 1.77 nM (PMID: 24942562). This antibody was also used in the direct and indirect immunofluorescence staining of frozen human skin sections (PMID: 24942562; 27304671) The IgG1 and IgG4 variants of this antibody induce loss of cell surface Dsg3 in primary human epidermal keratinocytes and suprabasal blisters in human skin (PMID: 27304671). This antibody is pathogenic in nature and caused suprabasal blisters in human skin at comparable doses of 25 μg and 50 μg (PMID: 27304671).

Product Class:	Purified
Clone ID:	F779
Synonyms:	DSG3; PVA; CDHF6; Desmoglein 3; 130 kDa pemphigus vulgaris antigen; Cadherin family member 6
Amount:	200 μg
Application Codes Clone:	ELISA; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds human desmoglein-3 between amino acids 1-161 and does not cross react with desmoglein-1. It is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Pemphigus vulgaris (PV) is a potentially lethal skin disease in which epidermal blisters occur as the result of the loss of cell-cell adhesion caused by the action of autoantibodies against desmoglein 3. It is characterized by suprabasal blistering of skin and mucous membranes.
Application Notes:	This antibody can be used for the determination of desmoglein-3 using ELISA. The binding affinity of IgG1 version of this antibody for human desmoglein-3 was determined using surface plasmon resonance and was found to be Kd = 1.77 nM (PMID: 24942562). This antibody was also used in the direct and indirect immunofluorescence staining of frozen human skin sections (PMID: 24942562; 27304671) The IgG1 and IgG4 variants of this antibody induce loss of cell surface Dsg3 in primary human epidermal keratinocytes and suprabasal blisters in human skin (PMID: 27304671). This antibody is pathogenic in nature and caused suprabasal blisters in human skin at comparable doses of 25 μg and 50 μg (PMID: 27304671).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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