Vector Labs Ab03423-21.0 Anti-Apical complex glycoprotein CSL [3E2], Mouse IgM, kappa (50 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 3E2
Synonyms: CSL; αCSL; Apical complex glycoprotein, CSL (circumsporozoite-like) antigen
Amount: 50 μg
Application Codes Clone: WB; ELISA; electron microscopy; therapeutic
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds the apical complex glycoprotein CSL of Cryptosporidium parvum. The apicomplexan protozoan Cryptosporidium parvum is a coccidian parasite that infects intestinal epithelium and causes diarrheal disease in humans and other agriculturally important food animals for which specific therapy and immunoprophylaxis are unavailable. Cryptosporidiosis is self-limiting in hosts with normal immune systems; however, in neonates, the elderly, and hosts having congenital or acquired immunodeficiency diseases or chemotherapy-induced immunosuppression, cryptosporidial enterocolitis may become chronic and have severe consequences.
Application Notes: Apical complex glycoprotein antigen of Cryptosporidium parvum is critical to infectivity and targets protective immunity. This antibody exhibited morphologic changes in sporozoites and merozoites characterized by rapid and progressive formation, posterior movement, and release of membranous Ag-mAb precipitates. This antibody was capable of recognizing 46-770 kDa sporozoite antigens and 1300 kDa antigen soluble glycoprotein exoantigen (CSL) expressed by merozoites in a western blot (PMID: 9029117). This antibody elicited circumsporozoite precipitate (CSP)-like reaction which is characterized by the progressive posterior formation and release of membranous antigen-MAb complexes, after which zoites are rendered noninfective. This antibody is capable of neutralizing C. parvum in vitro and also capable of passively protecting against oocyst challenge and infection in vivo. The identification of sporozoite-neutralizing antibody was done using ELISA (PMID: 10768951). This antibody was reported to be highly effective in in reducing, but not eliminating, persistent C. parvum infection (PMID: 11796330). This antibody in combination with PLA2 or LL37 was also used in the generation of a biocide fusion proteins to kill C. parvum sporozoites in vitro and in vivo (PMID: 20086143).

Product Class:	Purified
Clone ID:	3E2
Synonyms:	CSL; αCSL; Apical complex glycoprotein, CSL (circumsporozoite-like) antigen
Amount:	50 μg
Application Codes Clone:	WB; ELISA; electron microscopy; therapeutic
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds the apical complex glycoprotein CSL of Cryptosporidium parvum. The apicomplexan protozoan Cryptosporidium parvum is a coccidian parasite that infects intestinal epithelium and causes diarrheal disease in humans and other agriculturally important food animals for which specific therapy and immunoprophylaxis are unavailable. Cryptosporidiosis is self-limiting in hosts with normal immune systems; however, in neonates, the elderly, and hosts having congenital or acquired immunodeficiency diseases or chemotherapy-induced immunosuppression, cryptosporidial enterocolitis may become chronic and have severe consequences.
Application Notes:	Apical complex glycoprotein antigen of Cryptosporidium parvum is critical to infectivity and targets protective immunity. This antibody exhibited morphologic changes in sporozoites and merozoites characterized by rapid and progressive formation, posterior movement, and release of membranous Ag-mAb precipitates. This antibody was capable of recognizing 46-770 kDa sporozoite antigens and 1300 kDa antigen soluble glycoprotein exoantigen (CSL) expressed by merozoites in a western blot (PMID: 9029117). This antibody elicited circumsporozoite precipitate (CSP)-like reaction which is characterized by the progressive posterior formation and release of membranous antigen-MAb complexes, after which zoites are rendered noninfective. This antibody is capable of neutralizing C. parvum in vitro and also capable of passively protecting against oocyst challenge and infection in vivo. The identification of sporozoite-neutralizing antibody was done using ELISA (PMID: 10768951). This antibody was reported to be highly effective in in reducing, but not eliminating, persistent C. parvum infection (PMID: 11796330). This antibody in combination with PLA2 or LL37 was also used in the generation of a biocide fusion proteins to kill C. parvum sporozoites in vitro and in vivo (PMID: 20086143).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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