Mfg Part Number
Ab03425-1.1
Vector Labs Ab03425-1.1 Anti-HER2 [FRP5], Mouse IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing female BALB/c mice with intact SKBR3 human breast tumor cells.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | FRP5 |
| Synonyms: | CD340; NEU; HER-2; HER-2/neu; ERBB2; MLN 19; NGL; TKR1; erb-b2 receptor tyrosine kinase 2; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; FC; ELISA; agonist |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognizes an extracellular epitope of human HER2. |
| Application Notes: | This antibody has been successfully used to immunoprecipitate p185 erbB-2 protein from human SKBR3 breast tumor cell lysates and human erbB-2-transfected mouse HCll R111 cells. Furthermore, the binding of this antibody to surface-expressed HER2 on SKBR3 cells was determined using flow cytometry. Treatment with this antibody led to a rapid increase in the phosphotyrosine content of the erbB-2 protein in various cell lines. The binding of this antibody stimulated the turnover of the erbB-2 protein but did not stimulate DNA synthesis in HCll R111 cells. Therefore, it can be inferred that this antibody acts as a partial ligand agonist (Harwerth et al., 1992; PMID: 1353079). The binding affinity of this antibody for human HER2 was determined using ELISA (Wels et al., 1992; PMID: 1369487). The scFv version of this antibody was used in the generation of scFv(FRP5)-ETA, an antibody-exotoxin recombinant protein and tested for its anti-tumor activity (Harwerth et al., 1993; PMID: 7903153). ScFv(FRP5)-ETA displayed potent antitumoral activity in vitro against a wide range of established and primary human tumor cells, including breast and ovarian carcinomas, squamous cell carcinomas and prostate carcinomas. scFv(FRP5)-ETA also effectively inhibited the in vivo growth of human tumor xenografts and murine and rat tumor cells stably transfected with human c-erbB2 constructs (von Minckwitz et al., 2005; PMID: 16168106). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |