Vector Labs Ab03426-3.0 Anti-CD16 [NM3E2], Mouse IgG2b, lambda (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: NM3E2
Synonyms: CD16a; CD16b; FCGR3; FCGR3A; FCGR3B; FcγRIII; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; Fc-gamma RIII-beta; Fc-gamma RIIIb; FcRIIIb; Low affinity immunoglobulin gamma Fc region receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B
Amount: 200 μg
Application Codes Clone: ELISA; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds the extracellular domain of human CD16. This antibody recognizes an epitope in the vicinity of the Fc binding pocket. CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction.
Application Notes: This antibody is recommended for use in the generation of bispecific antibodies. The binding affinity of this antibody to human CD16 was determined using ELISA and surface plasmon resonance. The original scFv antibody binds human CD16 with an affinity of Kd= 0.2 nM. This antibody in combination with anti-HER2 antibody C6.5 was used in the generation of a bispecific antibody bsAb 2B1, which targets the extracellular domains (ECD) of the HER2/neu protooncogene product and the human FcgammaRIII or CD16. In the presence of the C6.5 x NM3E2 bs-scFv, peripheral blood lymphocytes promoted significant lysis of human SK-OV-3 ovarian cancer cells overexpressing HER2/neu (PMID:10449096). This antibody was also used in the generation of a heterodimeric bispecific scFv antibodies against EpCAM and CD16. This bispecific antibody was reported to enhance the killing of human carcinomas of the prostate, breast, colon, head, and neck even at very low effector: target ratios (PMID: 23611188).

Product Class:	Purified
Clone ID:	NM3E2
Synonyms:	CD16a; CD16b; FCGR3; FCGR3A; FCGR3B; FcγRIII; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; Fc-gamma RIII-beta; Fc-gamma RIIIb; FcRIIIb; Low affinity immunoglobulin gamma Fc region receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B
Amount:	200 μg
Application Codes Clone:	ELISA; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds the extracellular domain of human CD16. This antibody recognizes an epitope in the vicinity of the Fc binding pocket. CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction.
Application Notes:	This antibody is recommended for use in the generation of bispecific antibodies. The binding affinity of this antibody to human CD16 was determined using ELISA and surface plasmon resonance. The original scFv antibody binds human CD16 with an affinity of Kd= 0.2 nM. This antibody in combination with anti-HER2 antibody C6.5 was used in the generation of a bispecific antibody bsAb 2B1, which targets the extracellular domains (ECD) of the HER2/neu protooncogene product and the human FcgammaRIII or CD16. In the presence of the C6.5 x NM3E2 bs-scFv, peripheral blood lymphocytes promoted significant lysis of human SK-OV-3 ovarian cancer cells overexpressing HER2/neu (PMID:10449096). This antibody was also used in the generation of a heterodimeric bispecific scFv antibodies against EpCAM and CD16. This bispecific antibody was reported to enhance the killing of human carcinomas of the prostate, breast, colon, head, and neck even at very low effector: target ratios (PMID: 23611188).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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