Mfg Part Number
Ab03433-10.3
Vector Labs Ab03433-10.3 Anti-TIM-3 [mab15], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice or SJL mice with human and mouse TIM-3-Fc protein.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | mab15 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | CD366; HAVCR2; HAVcr-2; TIM3; TIMD-3; TIMD3; Hepatitis A virus cellular receptor 2; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3 |
| Amount: | 200 μg |
| Application Codes Clone: | inhibit; ELISA; FC; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds human TIM-3 and capable of inhibiting the interaction of TIM-3 and LILRB2. It is reported that this antibody strongly binds CC′ loop and mildly to the DE loop of TIM-3. Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein expressed on the cell surface of IFNγ producing CD4+ Th1 and CD8+ Tc1 cells. This protein is also expressed on the surface of Th17 cells, regulatory T-cells and innate immune cells like dendritic cells, NK cells and monocytes. It is a cell surface receptor implicated in modulating innate and adaptive immune responses (US20160200815). |
| Application Notes: | This antibody can inhibit the interaction between TIM-3 and LILRB2 and can be used in the treatment of TIM-3 related disorders. The binding characterization of this antibody to human and mouse TIM-3 was done using ELISA and flow cytometry. Blocking of TIM-3:LILRB2 interactions by this antibody was evaluated in activated-macrophage and activated-DC assays. This antibody was able to initiate TNFα secretion in LPS activated macrophages. The stimulation of pro-inflammatory cytokines GM-CSF, IL-1α, IL-1β, IL-6 and TNFα by this antibody was greater for macrophages from the LILRB2+ donor compared to the low LILRB2 donor |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |