Mfg Part Number
Ab03439-1.1
Vector Labs Ab03439-1.1 Anti-LAG-3 [ADI-31798], Mouse IgG1, kappa (200 μg)
Quick Overview
The original antibody was isolated from a yeast display library by two rounds of screening against human LAG-3.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | ADI-31798 |
| Synonyms: | CD223; LAG3; FDC; Lymphocyte activation gene 3 protein |
| Amount: | 200 μg |
| Application Codes Clone: | FC; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG4 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds human LAG-3. The human LAG-3 is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor on activated T-cells. |
| Application Notes: | This antibody can bind human LAG-3 in an ELISA. This antibody binds human LAG-3 with an affinity of Kd= 9.73 X 10^(-10) nM. The binding specificity of this antibody to human LAG-3 expressed on the surface of HEK293 and activated CD4+ T cells was done using flow cytometry. This antibody binds hLAG-3 expressed on the surface of HEK293 cells with an EC50 of 0.201 nM and activated CD4+ T cells with an EC50 of 0.00595 nM. This antibody can also effectively block the binding of LAG-3 and its ligand MHCII (HLA-DR) with an IC50 of 4.701 nM. The tumor suppressive effects of this antibody was studied in A375 human PBMC model (CN112166125). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |