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Mfg Part Number

Ab03440-2.0

Vector Labs Ab03440-2.0 Anti-Hexon [9C12], Mouse IgG2a, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing mice with Ad type 5 (Ad5).

$570.70

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Description

Product Class: Purified
Clone ID: 9C12
Synonyms: Hexon protein; CP-H; Protein II
Amount: 100 μg
Application Codes Clone: WB; IP; Neutralizing; Blocking; cryo-EM; ELISA; SPR; Crystallization; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody binds hexon protein from Ad5 particles. In particular, it binds to HVRs 2, 5 and 7 of the hexon. Hexon is the major coat protein of adenovirus.
Application Notes: The antibody was able to neutralize Ad5. Cryo-EM reconstruction of the Ad5-9C12 complex showed that the capsid is coated by a meshwork of bivalently bound antibody molecules. The antibody bound to the hexon protein of Ad5 particles denatured as shown by western blot analysis (Varghese et al., 2004; PMID: 15507619).The crystal structure of scFv fragment of the antibody in complex with human adenovirus C5 hexon was determined (Schmid et al., 2018; PMID: 29386504). The specificity of the original format of the antibody was confirmed by ELISA analysis (Moyer et al., 2016; PMID: 26491163). Wild-type viruses were immunoprecipitated using the antibody (Myerset al., 2013; PMID: 23487468). The binding affinity of the Fab fragment to Adv5 hexon was measured by surface plasmon resonance (Kd= 1 nM) (Bottermann et al., 2016; PMID: 27881870). The antibody detected hexon on A549 cells by immunofluorescence analysis (Smith et al., 2008; PMID: 18448546).

Product Class:	Purified
Clone ID:	9C12
Synonyms:	Hexon protein; CP-H; Protein II
Amount:	100 μg
Application Codes Clone:	WB; IP; Neutralizing; Blocking; cryo-EM; ELISA; SPR; Crystallization; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody binds hexon protein from Ad5 particles. In particular, it binds to HVRs 2, 5 and 7 of the hexon. Hexon is the major coat protein of adenovirus.
Application Notes:	The antibody was able to neutralize Ad5. Cryo-EM reconstruction of the Ad5-9C12 complex showed that the capsid is coated by a meshwork of bivalently bound antibody molecules. The antibody bound to the hexon protein of Ad5 particles denatured as shown by western blot analysis (Varghese et al., 2004; PMID: 15507619).The crystal structure of scFv fragment of the antibody in complex with human adenovirus C5 hexon was determined (Schmid et al., 2018; PMID: 29386504). The specificity of the original format of the antibody was confirmed by ELISA analysis (Moyer et al., 2016; PMID: 26491163). Wild-type viruses were immunoprecipitated using the antibody (Myerset al., 2013; PMID: 23487468). The binding affinity of the Fab fragment to Adv5 hexon was measured by surface plasmon resonance (Kd= 1 nM) (Bottermann et al., 2016; PMID: 27881870). The antibody detected hexon on A549 cells by immunofluorescence analysis (Smith et al., 2008; PMID: 18448546).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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