Mfg Part Number
Ab03446-30.11
Vector Labs Ab03446-30.11 Anti-spike protein [TZZ14], scFv fragment (His), ScFv, (50 μg)
Quick Overview
A scFv antibody phage display library was constructed from peripheral blood lymphocytes of piglets infected with TGEV. The library was screened with four rounds of biopanning using purifed TGEV antigen, and scFv antibodies that bound to TGEV were obtained.
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| Product Class: | Purified |
|---|---|
| Clone ID: | TZZ14 |
| Heavy Chain Modification: | ScFv |
| Synonyms: | TGEV; Spike glycoprotein; S glycoprotein; E2; Peplomer protein |
| Amount: | 50 μg |
| Application Codes Clone: | in vitro; IF; WB; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for the S protein of TGEV. The antibody does not show any cross-reactivity with other available porcine pathogens, such as PEDV, PRRSV, PCV2. |
| Application Notes: | To determine whether scFv antibody neutralized TGEV infection, a PRN assay was performed. The results showed that the antibody signifcantly inhibited TGEV infection in a dose-dependent manner and completely neutralized virus replication at a dilution of 1:40. Therefore the antibody showed a neutralization efect on TGEV infection. The specificity of the scFv format of the antibody was confirmed by ELISA analysis. The antibody detected the S protein by western blot analysis. IFA results showed that the antibody bound to ST TGEV-infected cells and to TGEV S protein-transfected cells (Zhang et al., 2019; PMID: 30729994). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |