Vector Labs Ab03450-23.0 Anti-beta 1 integrin [AIIB2], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: AIIB2
Synonyms: integrin beta 1; Integrin beta-1-binding protein 1; Integrin cytoplasmic domain-associated protein 1 (ICAP-1)
Amount: 200 μg
Application Codes Clone: in vivo; inhibition; FACS; IP; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody specifically binds β1 integrin extracellular domain.
Application Notes: The antibody immunoprecipitated the integrin subunits (Hall et al.,1990). This antibody was used for detection of β1 integrin expressed on CHO cells by flow cytometry (Takada et al., 1993; PMID: 7688727). The original format of the antibody or its F(ab′)2 fragments applied to single cells were capable of down-modulating β1 integrin signaling pathways (Wang et al., 1998; PMID: 9843973 and Weaver et al., 1997; PMID: 9105051). The antibody was used in immunofluorescence characterization of integrins in the HMT-3522 cells (Weaver et al., 1997; PMID: 9105051). The antibody inhibited tumor cell growth in several breast cancer cell lines (T4-2, MDA-MB-231, BT474, SKBR3, and MCF-7) and one nonmalignant cell line (S-1). Further, β1 integrin inhibition resulted in a significant loss of cancer cells, associated with a decrease in proliferation and increase in apoptosis, and a global change in the composition of residual colonies (Park et al., 2010; PMID: 16452209). The antibody was found to reduce the frequency of solid tumor stem cells. The antibody inhibited the growth of colon tumors and reduced the percentage of CD44+ cells. Tumors treated in mice showed a decrease when treated with the antibody. The humanized version of the antibody was constructed, showing potential for therapeutic uses (WO2009009114A3).

Product Class:	Purified
Clone ID:	AIIB2
Synonyms:	integrin beta 1; Integrin beta-1-binding protein 1; Integrin cytoplasmic domain-associated protein 1 (ICAP-1)
Amount:	200 μg
Application Codes Clone:	in vivo; inhibition; FACS; IP; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody specifically binds β1 integrin extracellular domain.
Application Notes:	The antibody immunoprecipitated the integrin subunits (Hall et al.,1990). This antibody was used for detection of β1 integrin expressed on CHO cells by flow cytometry (Takada et al., 1993; PMID: 7688727). The original format of the antibody or its F(ab′)2 fragments applied to single cells were capable of down-modulating β1 integrin signaling pathways (Wang et al., 1998; PMID: 9843973 and Weaver et al., 1997; PMID: 9105051). The antibody was used in immunofluorescence characterization of integrins in the HMT-3522 cells (Weaver et al., 1997; PMID: 9105051). The antibody inhibited tumor cell growth in several breast cancer cell lines (T4-2, MDA-MB-231, BT474, SKBR3, and MCF-7) and one nonmalignant cell line (S-1). Further, β1 integrin inhibition resulted in a significant loss of cancer cells, associated with a decrease in proliferation and increase in apoptosis, and a global change in the composition of residual colonies (Park et al., 2010; PMID: 16452209). The antibody was found to reduce the frequency of solid tumor stem cells. The antibody inhibited the growth of colon tumors and reduced the percentage of CD44+ cells. Tumors treated in mice showed a decrease when treated with the antibody. The humanized version of the antibody was constructed, showing potential for therapeutic uses (WO2009009114A3).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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