Mfg Part Number
Ab03454-10.3
Vector Labs Ab03454-10.3 Anti-LAG-3 [Ab2317], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
Vector Labs Ab03454-10.3 Anti-LAG-3 [Ab2317], Human IgG1, Fc Silent™, kappa (200 μg)
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | Ab2317 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | CD223; LAG3; FDC; Lymphocyte activation gene 3 protein |
| Amount: | 200 μg |
| Application Codes Clone: | SPR; blocking; in vivo |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG4 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG4 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody is specific for human LAG-3. The antibody does not detect cynomolgus LAG-3 or murine LAG-3. |
| Application Notes: | The antibody is a humanized version of mab23. The antibody had an activity EC50 of 0.34 μg/ml for activating human T cells to release IL-2. Further, the antibody activates human DC cell to stimulate human T cell activity (EC50 of 0.21nM), as shown by MLR assay. The humanized antibody efficacy in vivo was evaluated of the present invention. Tumors treated in mice showed an inhibition in tumor growth when treated with a combination of the antibody and PD-1 antibody. The binding affinity of the antibody to hLAG-3 was measured by surface plasmon resonance (Kd= 0.2 nM). The antibody showed a good blocking activity. The antibody was used in combination with an anti PD-1 antibody (Nivo or Pem) into a bispecific antibody (CN112079925). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |