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Mfg Part Number

Ab03463-23.0

Vector Labs Ab03463-23.0 Anti-Tissue factor [1157], Rabbit IgG, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing 6-week-old Wistar female rats intraperitoneally with recombinant mouse tissue factor protein comprising amino acid sequence between 30 to 251 in Freund's complete adjuvant.

$570.70

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Description

Product Class: Purified
Clone ID: 1157
Synonyms: CD142; TF; mTF; F3; Coagulation factor III; Thromboplastin; platelet tissue factor; factor III
Amount: 100 μg
Application Codes Clone: ELISA; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds mouse tissue factor (mTF) between amino acids 30 to 251. mTF hemostatic protein which is involved in the extrinsic pathway of coagulation. TF is receptor for circulating serine protease factor VIIa, activating the catalytic VIIa protease domain. The complex formed functions to activate the zymogen factor X into activated factor Xa, which then catalyzes the formation of Prothrombin to Thrombin. The pathways results in a fibrin clot which acts to stop bleeding. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Application Notes: The initial binding characterization of the hybridoma supernatant for mouse tissue factor was done using ELISA. The binding was further confirmed with flow cytometry by measuring the reactivity of the hybridoma culture supernatant to mTF-expressing cells. This antibody was capable of being internalized by TF-forced-expression mouse B16 melanoma cells expressing mTF (US9920133).

Product Class:	Purified
Clone ID:	1157
Synonyms:	CD142; TF; mTF; F3; Coagulation factor III; Thromboplastin; platelet tissue factor; factor III
Amount:	100 μg
Application Codes Clone:	ELISA; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds mouse tissue factor (mTF) between amino acids 30 to 251. mTF hemostatic protein which is involved in the extrinsic pathway of coagulation. TF is receptor for circulating serine protease factor VIIa, activating the catalytic VIIa protease domain. The complex formed functions to activate the zymogen factor X into activated factor Xa, which then catalyzes the formation of Prothrombin to Thrombin. The pathways results in a fibrin clot which acts to stop bleeding. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Application Notes:	The initial binding characterization of the hybridoma supernatant for mouse tissue factor was done using ELISA. The binding was further confirmed with flow cytometry by measuring the reactivity of the hybridoma culture supernatant to mTF-expressing cells. This antibody was capable of being internalized by TF-forced-expression mouse B16 melanoma cells expressing mTF (US9920133).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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