Mfg Part Number
Ab03473-10.3

Vector Labs Ab03473-10.3 Anti-Protein C [IgG1-7], Human IgG1, Fc Silent™, kappa (100 μg)

Quick Overview
The original antibody was generated from the original mouse antibody HPC-4 by performing site directed mutagenesis, consensus and resurfacing methods.
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Product Class:Purified
Clone ID:IgG1-7
Heavy Chain Modification:Fc Silent™
Synonyms:PROC; Vitamin K-dependent protein C; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV; HPC-4; HPC4; Fab-7
Amount:100 μg
Application Codes Clone:ELISA; Blocking; Purification
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:This antibody specifically recognizes and binds the ProC epitope tag comprising the amino acid sequence 'EDQVDPRLIDGK'. This antibody binds the zymogen form of human Protein C in the activation peptide (spanning the thrombin cleavage site) and blocks protein C activation by thrombin–thrombomodulin both in vitro and in vivo. It does not react with activated protein C.
Application Notes:This antibody is the humanized version of anti-Protein C antibody HPC-4. The binding of this antibody to protein C is calcium dependent. This humanized anti-protein C antibody exhibited calcium dependent binding which was significantly increased over that of the murine antibody HPC-4. The binding characterization of this antibody was performed using ELISA (PMID: 9680194). This antibody can be used to purify protein tagged with this 'EDQVDPRLIDGK' epitope.
More Information
Brand Vector Labs
UNSPSC 12161500