Mfg Part Number
Ab03476-1.159-BS
Vector Labs Ab03476-1.159-BS Anti-mCherry [LAM-4], Mouse IgG1-Fc Fusion, (1 mg)
Quick Overview
The original antibody was generated by immunization of Llama glama with mCherry fluorescent protein. A VHH cDNA library was generated by high-throughput DNA sequencing of a marrow lymphocyte from an immunized llama. Later on this was combined with mass spectrometric (MS) identification of high affinity VHH regions derived from serum of the same animal.
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| Product Class: | Purified |
|---|---|
| Clone ID: | LAM-4 |
| Synonyms: | mCh; RFP; Fluorescent protein raspberry; Red fluorescent proteins; MCherry fluorescent protein; DsRed; RFP-RANbody-4; LaM4; RFP nanobody; RNb |
| Amount: | 1 mg |
| Application Codes Clone: | ELISA; IF; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS only. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds mCherry and can also bind DsRed. |
| Application Notes: | This antibody can be used to detect mCherry fluorescent protein and DsRed in an ELISA. This antibody binds immobilized mCherry with a binding affinity of Kd= 0.18 nM. The Alexa Fluor® 488 conjugated version of this antibody was used to immunofluorescently stain S. cerevisiae expressing mCherry-tagged histone H2B (PMID: 25362362). This antibody was used for the visualization and manipulation of red fluorescent labelled proteins in live cells by using nanobodies targeting fluorescent protein tags (PMID: 31122229). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |