Mfg Part Number
Ab03494-23.0

Vector Labs Ab03494-23.0 Anti-Alpha-Fetoprotein [AB3], Rabbit IgG, kappa (100 μg)

Quick Overview
The original antibody was generated by immunizing BALB/c mice with human alpha-fetoprotein.
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Product Class:Purified
Clone ID:AB3
Synonyms:AFP; Alpha-fetoglobulin; A-Feto-protein
Amount:100 μg
Application Codes Clone:ELISA; IF; IHC
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:This antibody binds human alpha-fetoprotein (AFP). AFP is made in the liver of developing babies. It binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. AFP levels in healthy adults is very low. Elevated levels of AFP in adult human blood is a marker for cancer.
Application Notes:This antibody can be used in combination with another anti-alpha fetoprotein antibody AB2 for detection of human AFP using sandwich ELISA. AB3 is used as the capture antibody and HRP labelled AB2 is used as detection antibody. This antibody was also used for the immunofluorescent staining of HepG2 hepatoma cells and immunohistochemical analysis of human liver cancer tissue (CN107022029).
More Information
Brand Vector Labs
UNSPSC 12161500