Mfg Part Number
Ab03497-10.0
Vector Labs Ab03497-10.0 Anti-Digoxigenin B [Clone 2], Human IgG1, lambda (100 μg)
Quick Overview
The original antibody was isolated from a IgM-derived phage antibody library created from the peripheral blood lymphocytes of 20 non-immunized doners. The specific clone was isolated by panning the library against digoxigenin.
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| Product Class: | Purified |
|---|---|
| Clone ID: | Clone 2 |
| Synonyms: | Digoxigenin; DigB; DOG; ChEBI: 42098; CAS:1672-46-4 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length reformatted human antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody is specific for digoxigenin A. Digoxigenin (DIG) is a hydroxy steroid that consists of 5beta-cardanolide, found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata. It is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications. |
| Application Notes: | The binding specificity of this antibody for digoxigenin was checked using ELISA. This antibody was able to bind digoxigenin coupled by a methylcarbonyl (Dig-Met) or succinyl (Dig-Suc) type linker to BSA. The presence of Dig-dUTP conjugate resulted in competitive inhibition on binding of this antibody to digoxigenin coupled with methylcarbonyl (Dig-Met) liked to BSA (PMID: 9305722). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |