Mfg Part Number
Ab03503-23.159
Vector Labs Ab03503-23.159 Anti-Beta Lactamase [cAbTEM13], Rabbit IgG-Fc Fusion, (100 μg)
Quick Overview
The original antibody was generated by immunizing adult dromedary (Camelus dromedarius) with TEM-1 β-lactamase.
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| Product Class: | Purified |
|---|---|
| Clone ID: | cAbTEM13 |
| Synonyms: | β-Lactamase; bla; Beta-lactamase TEM; IRT-4; Penicillinase; TEM-1 β-lactamase; cAb-TEM13 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; inhibit |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 11557473 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds an enzyme called beta-lactamase produced by bacteria, which catalysis the breaking of the beta-lactam ring of beta-lactam antibiotics such as penicillin, cephalosporin, cephamycin and monobactam through hydrolysis. The breaking of the beta-lactam ring deactivates the antibacterial properties of the antibiotic molecule and provides antibiotic resistance in bacteria. |
| Application Notes: | The binding reactivity of this antibody to TEM-1 beta-lactamase was determined using ELISA. This antibody was capable of inhibiting beta-lactamase activity in vitro with an IC50 of 0.08 μM. The in vivo inhibition activity of this antibody to inhibit surface-exposed TEM-1 and prevent bacterial growth was assessed by preincubating cells expressing TEM-1 with increasing amounts of the antibody fragment. It was reported that when cells were preincubated with cAbTem13 before plating lost their ability to neutralize the antibiotic ampicillin (PMID: 11557473). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |