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Mfg Part Number

Ab03504-10.0

Vector Labs Ab03504-10.0 Anti-Clusterin [16B5], Human IgG1, kappa (100 μg)

VECTOR LABS

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Vector Labs Ab03504-10.0 Anti-Clusterin [16B5], Human IgG1, kappa (100 μg)

$570.70

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Description

Product Class: Purified
Clone ID: 16B5
Synonyms: CLU; APOJ; CLI; KUB1; AAG4
Amount: 100 μg
Application Codes Clone: IF; in vitro; in vivo; inhibition
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody binds clusterin on the EMT-epitope.
Application Notes: The effect of the original format of the antibody was evaluated on lung metastasis in BALB/c mice with orthotopically implanted 4T1 cells. The antibody was able to significantly reduce lung metastasis (Lenferink et al., 2010; PMID: 19935703). A humanized version of the antibody was constructed. The humanized version of the antibody inhibited the EMT-inducing activity of clusterin on A549 NSCLC cells. Immunofluorescence was performed on A549 cells using this antibody to monitor E-cadherin expression. The combination of the humanized version of the antibody with erlotinib resulted in an enhanced anti-tumour effect (H1299 and H460 cells) (US9822170). AB-16B5 is a humanized IgG2 version of the antibody which specifically binds to tumour-associated clusterin and inhibits its activity. This antibody showed a good tumour inhibitory effect in in vivo application (Tremblay et al.; 2010). A phase I and phase II trial to assess safety and tolerability of AB-16B5 for the treatment of solid tumours was performed (Ferrario et al., 2017; NCT04364620).

Product Class:	Purified
Clone ID:	16B5
Synonyms:	CLU; APOJ; CLI; KUB1; AAG4
Amount:	100 μg
Application Codes Clone:	IF; in vitro; in vivo; inhibition
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody binds clusterin on the EMT-epitope.
Application Notes:	The effect of the original format of the antibody was evaluated on lung metastasis in BALB/c mice with orthotopically implanted 4T1 cells. The antibody was able to significantly reduce lung metastasis (Lenferink et al., 2010; PMID: 19935703). A humanized version of the antibody was constructed. The humanized version of the antibody inhibited the EMT-inducing activity of clusterin on A549 NSCLC cells. Immunofluorescence was performed on A549 cells using this antibody to monitor E-cadherin expression. The combination of the humanized version of the antibody with erlotinib resulted in an enhanced anti-tumour effect (H1299 and H460 cells) (US9822170). AB-16B5 is a humanized IgG2 version of the antibody which specifically binds to tumour-associated clusterin and inhibits its activity. This antibody showed a good tumour inhibitory effect in in vivo application (Tremblay et al.; 2010). A phase I and phase II trial to assess safety and tolerability of AB-16B5 for the treatment of solid tumours was performed (Ferrario et al., 2017; NCT04364620).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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