Mfg Part Number
Ab03506-10.3
Vector Labs Ab03506-10.3 Anti-TIGIT [MT251], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with recombinant human TIGIT protein.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | MT251 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | VSTM3; VSIG9; WUCAM; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain-containing protein 3 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; FACS; blocking |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The original antibody is specific for TIGIT. The antibody does not cross-react with LAG-3, PD-1 and PD-L1. |
| Application Notes: | The original mouse antibody was subjected to humanized modification, keeping a similar binding activity. The specificity of the original and the humanized format of the antibody was confirmed by ELISA analysis (EC50= 0.09901 μg/mL and 0.07412 μg/mL respectively). The original format of antibody blocked the binding activity of TIGIT and CD155. The original format and the humanized version of the antibody could recognize the TIGIT antigen expressed by Jurkat/TIGIT cells by FACS analysis. The original format of the antibody was able to effectively block the binding of TIGIT to CD155 (CN112794909). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |