Mfg Part Number
Ab03509-23.0
Vector Labs Ab03509-23.0 Anti-LAG-3 [Ab835], Rabbit IgG, kappa (100 μg)
Quick Overview
Vector Labs Ab03509-23.0 Anti-LAG-3 [Ab835], Rabbit IgG, kappa (100 μg)
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | Ab835 |
| Synonyms: | CD223; LAG3; FDC; Lymphocyte activation gene 3 protein |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG4 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG4 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody is specific for human LAG-3. The antibody does not cross react with cynomolgus LAG-3. |
| Application Notes: | The antibody is a humanized version of the mouse antibody mab7c. The specificity of the original format of the antibody was confirmed by ELISA analysis (EC50=0.12 nM). The binding activity of the humanized antibody was tested using the hLAG-3 positive cell line, and the results showed that the antibody was positive for hLAG-3. The binding activity of the antibody and hLAG-3 positive cells was EC50 = 0.41 nM. The binding affinity of the antibody to hLAG-3 was measured by surface plasmon resonance (Kd= 0.52 nM). The antibody blocked the binding of MHCII and human LAG-3 with IC50 = 2.27 nM and it prevented LAG-3 and MHCII from binding to activate human T cell activity (release IL2) (EC50=1.74ug/ml) (CN110172099). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |