Mfg Part Number
Ab03511-10.3
Vector Labs Ab03511-10.3 Anti-LAG-3 [B5D1], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
Vector Labs Ab03511-10.3 Anti-LAG-3 [B5D1], Human IgG1, Fc Silent™, kappa (100 μg)
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | B5D1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | CD223; LAG3; FDC; Lymphocyte activation gene 3 protein |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; SPR; FC; blocking; in vitro |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody is specific for human LAG-3. The antibody cross-reacts with cynomolgus LAG-3. |
| Application Notes: | The humanized version of the antibody was constructed. The specificity of the original format of the antibody was confirmed by ELISA analysis. The binding activity of the humanized antibody to LAG3 was EC 50= 63.3 ng/ml. The binding affinity of the humanized antibody was measured by surface plasmon resonance (Kd= 6.10^-10 M). This antibody was used for detection of LAG-3 expressed on CHO cells by flow cytometry. The antibody blocked the binding of LAG-3 antigen and MHC-II molecule (IC50= 0.152 ug/ml). The antibody could effectively activate the activity of antigen-specific T cells, EC50 of in vitro activity test results of B5D1 antibody equal to 1.25 ug/ml (CN110606892). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |