Mfg Part Number
Ab03524-10.29
Vector Labs Ab03524-10.29 Anti-F protein [3B1], Human Fab fragment, His-Tagged, kappa (100 μg)
Quick Overview
A synthetic, fully human antibody library was used for panning against RSV prefusion and post fusion F proteins. The original antibody against RSV F protein was discovered following phage library panning and characterized.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 3B1 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | fusion glycoprotein; Fusion glycoprotein F0 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The original antibody is specific for post fusion RSV F protein (amino acids D385, F387 and N388). F-protein plays a pivotal role in the pathogenesis of RSV by mediating the fusion between the viral and the host cell membrane. F protein exists in two distinct conformations: the metastable prefusion conformation and the stable post fusion conformation. |
| Application Notes: | The specificity of the Fab and IgG1 format of the antibody was confirmed by ELISA analysis (EC50 value for IgG1 format at 3.6 ng/mL). The binding affinity of the Fab fragment to post fusion form of F was measured by surface plasmon resonance (Kd= 68 nM). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |