Vector Labs Ab03531-1.159 Anti-S protein (RBD) [aRBD-2], Mouse IgG1-Fc Fusion, (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: aRBD-2
Synonyms: SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV; SARS-CoV2
Amount: 100 μg
Application Codes Clone: ELISA; blocking; SPR; neutralizing
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: VHH
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: The antibody binds the Spike protein of the SARS-CoV-2.
Application Notes: The VHH and VHH with IgG1 Fc formats demonstrated strong binding to both RBD and the entire ectodomain (S1+S2) of SARS-CoV-2 spike in ELISA, with a low nanomolar 50% effective concentration (EC50 respectively 1.338 nM and 1.521 nM). The binding affinity of the VHH format and VHH-Fc formats to RBD were also measured using surface plasmon resonance, with KD values of 2.60 nM and 1.57 nM respectively. Both VHH and Fc fusion formats could block RBD-ACE2 interaction in a dose-dependent manner, as characterized by competitive ELISA. Furthermore, the fusion of two VHHs with nonoverlapping epitopes resulted in two hetero-bivalent VHHs, aRBD-2-5 and aRBD-2-7, showed significantly higher RBD binding affinities (KD of 59.2 pM and 0.25 nM). Similarly, their Fc fusions also showed enhanced binding affinities, with KD values of 12.3 pM and 0.22 nM, respectively. The homo- and hetero-bivalent VHHs exhibited potent neutralizing ability against SARSCoV-2 inoculated onto Vero E6 cells. The 50% neutralization doses were 0.092mg/ml (1.12 nM) for aRBD-2-Fc, 1.22 ng/ml (0.043 nM) for aRBD-2-5 and 3.18 ng/ml (0.111 nM) for aRBD-2-7. Finally, the ND50s for aRBD-2-5-Fc and aRBD-2-7-Fc were 11.8 ng/ml (0.107 nM) and 6.76 ng/ml (0.0606 nM), respectively (Ma et al, 2021; PMID:33658349).

Product Class:	Purified
Clone ID:	aRBD-2
Synonyms:	SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV; SARS-CoV2
Amount:	100 μg
Application Codes Clone:	ELISA; blocking; SPR; neutralizing
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	VHH
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	The antibody binds the Spike protein of the SARS-CoV-2.
Application Notes:	The VHH and VHH with IgG1 Fc formats demonstrated strong binding to both RBD and the entire ectodomain (S1+S2) of SARS-CoV-2 spike in ELISA, with a low nanomolar 50% effective concentration (EC50 respectively 1.338 nM and 1.521 nM). The binding affinity of the VHH format and VHH-Fc formats to RBD were also measured using surface plasmon resonance, with KD values of 2.60 nM and 1.57 nM respectively. Both VHH and Fc fusion formats could block RBD-ACE2 interaction in a dose-dependent manner, as characterized by competitive ELISA. Furthermore, the fusion of two VHHs with nonoverlapping epitopes resulted in two hetero-bivalent VHHs, aRBD-2-5 and aRBD-2-7, showed significantly higher RBD binding affinities (KD of 59.2 pM and 0.25 nM). Similarly, their Fc fusions also showed enhanced binding affinities, with KD values of 12.3 pM and 0.22 nM, respectively. The homo- and hetero-bivalent VHHs exhibited potent neutralizing ability against SARSCoV-2 inoculated onto Vero E6 cells. The 50% neutralization doses were 0.092mg/ml (1.12 nM) for aRBD-2-Fc, 1.22 ng/ml (0.043 nM) for aRBD-2-5 and 3.18 ng/ml (0.111 nM) for aRBD-2-7. Finally, the ND50s for aRBD-2-5-Fc and aRBD-2-7-Fc were 11.8 ng/ml (0.107 nM) and 6.76 ng/ml (0.0606 nM), respectively (Ma et al, 2021; PMID:33658349).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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