Vector Labs Ab03533-1.159 Anti-S protein (RBD) [aRBD-7], Mouse IgG1-Fc Fusion, (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: aRBD-7
Synonyms: SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV; SARS-CoV2
Amount: 100 μg
Application Codes Clone: ELISA; blocking; SPR; neutralizing
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: VHH
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: The antibody binds the Spike protein of the SARS-CoV-2.
Application Notes: The VHH with IgG1 Fc formats demonstrated strong binding to both RBD and the entire ectodomain (S1+S2) of SARS-CoV-2 spike in ELISA, with a low nanomolar 50% effective concentration (EC50 respectively 0.522 nM and 0.748 nM). The binding affinity of the VHH format and VHH-Fc formats to RBD were also measured using surface plasmon resonance, with KD values of 3.31 nM and 72 pM respectively. Both VHH and Fc fusion formats could block RBD-ACE2 interaction in a dose-dependent manner, as characterized by competitive ELISA. Furthermore, the fusion of two VHHs with nonoverlapping epitopes resulted in the hetero-bivalent VHHs, aRBD-2-7, which showed significantly higher RBD binding affinities (KD of 0.25 nM). Similarly, their Fc fusions also showed enhanced binding affinities, with a KD value of 0.22 nM. The homo- and hetero-bivalent VHHs exhibited potent neutralizing ability against SARSCoV-2 inoculated onto Vero E6 cells. The 50% neutralization dose was 3.18 ng/ml (0.111 nM), for aRBD-2-7. Finally, the ND50s for aRBD-2-7-Fc was 6.76 ng/ml (0.0606 nM) (Ma et al, 2021; PMID:33658349).

Product Class:	Purified
Clone ID:	aRBD-7
Synonyms:	SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV; SARS-CoV2
Amount:	100 μg
Application Codes Clone:	ELISA; blocking; SPR; neutralizing
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	VHH
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	The antibody binds the Spike protein of the SARS-CoV-2.
Application Notes:	The VHH with IgG1 Fc formats demonstrated strong binding to both RBD and the entire ectodomain (S1+S2) of SARS-CoV-2 spike in ELISA, with a low nanomolar 50% effective concentration (EC50 respectively 0.522 nM and 0.748 nM). The binding affinity of the VHH format and VHH-Fc formats to RBD were also measured using surface plasmon resonance, with KD values of 3.31 nM and 72 pM respectively. Both VHH and Fc fusion formats could block RBD-ACE2 interaction in a dose-dependent manner, as characterized by competitive ELISA. Furthermore, the fusion of two VHHs with nonoverlapping epitopes resulted in the hetero-bivalent VHHs, aRBD-2-7, which showed significantly higher RBD binding affinities (KD of 0.25 nM). Similarly, their Fc fusions also showed enhanced binding affinities, with a KD value of 0.22 nM. The homo- and hetero-bivalent VHHs exhibited potent neutralizing ability against SARSCoV-2 inoculated onto Vero E6 cells. The 50% neutralization dose was 3.18 ng/ml (0.111 nM), for aRBD-2-7. Finally, the ND50s for aRBD-2-7-Fc was 6.76 ng/ml (0.0606 nM) (Ma et al, 2021; PMID:33658349).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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