Mfg Part Number
Ab03535-23.0
Vector Labs Ab03535-23.0 Anti-NP [JS08], Rabbit IgG, lambda (100 μg)
Quick Overview
A combinatorial fragment of antigen-binding (Fab) antibody phage library based on peripheral blood-derived from five coronavirus disease 2019 (COVID-19) infected donors was constructed. The antibody was obtained and identified by panning with purified NP.
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| Product Class: | Purified |
|---|---|
| Clone ID: | JS08 |
| Synonyms: | NP; NC; Protein N; Nucleocapsid protein; SARS-CoV Protein N; SARS-CoV Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; WB; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody is specific for NP. |
| Application Notes: | The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected the recombinant NP by western blot analysis. Surface plasmon resonance assay was performed to evaluate the binding specificity and affinity between antibodies and purified NP (KD in the sub-nM range). The antibody was used together with JS16 in sandwich ELISA (detection limit below 0.78ng/mL) (Zhang et al, 2020; PMID:33281824). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |