Mfg Part Number
Ab03554-10.3
Vector Labs Ab03554-10.3 Anti-F protein [mAb 338], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with a combination of some or all of the following: intranasal infection with hMPV at 10^6 TCID50 per animal of either NL1700, NL100, or NL199; intranasal infection with 10^6 PFU b/hPIV3/hMPV FNL100; intraperitoneal injection with adenovirus-vectored hMPV FNL100 or hMPV FNL199 at a dose of 9 × 10^7 TCID50; purified soluble hMPV F protein derived from NL100 and NL199 sequences injected intraperitoneally with either GERBU MM adjuvant.
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| Product Class: | Purified |
|---|---|
| Clone ID: | mAb 338 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Fusion glycoprotein; Protein F; hMPV F protein; hMPV fusion glycoprotein; Human metapneumovirus; 168-A5-338-284 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; neutralization |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Alpaca VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes the hMPV F protein (a.a. 238-245). Human metapneumovirus (hMPV) is a member of the Paramyxoviridae family/Pneumovirinae subfamily and shares many common features with respiratory syncytial virus (RSV), another member of the same subfamily. hMPV causes respiratory tract illnesses that, similar to human RSV, occur predominantly during the winter months and have symptoms that range from mild to severe cough, bronchiolitis, and pneumonia. |
| Application Notes: | ELISA was used to confirm the binding of this antibody to hMPV F protein. This antibody has been reported to have high neutralizing activity against all of the hMPV types A1, A2, B1, and B2 with an in vitro IC50 of < 2µg/ml. The antibody is effective as passive prophylaxis in protecting hamsters from HMPV challenge and also reduces incidence of MPV infection and disease in animal models (PMID: 16873237). Intraperitoneal injection of thia antibody into BALB/c mice significantly decreased lung viral titers after HMPV challenge when the antibody was administered prophylactically or therapeutically (PMID: PMID: 20619294). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |