Mfg Part Number
Ab03557-1.1
Vector Labs Ab03557-1.1 Anti-IL-1A [6H3.1A4], Mouse IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing mice with recombinant human IL-1 alpha.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | 6H3.1A4 |
| Synonyms: | IL1A; IL-1α; IL1F1; Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; 6H3.1A4.3E11 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds human Interleukin-1 alpha, which is a cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. |
| Application Notes: | The original mouse IgG antibody binds human IL-1A with a binding affinity of Kd= 0.35 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 2.40E−10M. This antibody was used in combination with 6B12.4F6 (anti-IL-1β) antibody for the generation of anti-human IL-1α/β DVD-Ig molecule that had therapeutic potential (PMID: 20068402). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |