Mfg Part Number
Ab03560-1.1
Vector Labs Ab03560-1.1 Anti-IL-1B [10G11], Mouse IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing IL-1α/β double KO mice with mouse IL-1 beta.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | 10G11 |
| Synonyms: | IL1B; IL-1β; Interleukin-1 beta; IL-1 beta |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds mouse Interleukin-1 beta, which is a potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. It promotes Th17 differentiation of T-cells and synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. It also plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. |
| Application Notes: | The original mouse IgG antibody binds mouse IL-1B with a binding affinity of Kd= 0.23 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 3.70E−10M. This antibody was used in combination with 9H10 (anti- mouse IL-1A) antibody for the generation of anti-mouse IL-1α/β DVD-Ig molecule (US7612181). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |