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Mfg Part Number

Ab03561-10.0

Vector Labs Ab03561-10.0 Anti-IL-1B [6B12.4F6], Human IgG1, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing mice with recombinant human IL-1 beta.

$570.70

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Description

Product Class: Purified
Clone ID: 6B12.4F6
Synonyms: IL1B; IL-1β; 1L1F2; Interleukin-1 beta; IL-1 beta; Catabolin
Amount: 100 μg
Application Codes Clone: ELISA; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds human Interleukin-1 beta, which is a potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. It promotes Th17 differentiation of T-cells and synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. It also plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Application Notes: The original mouse IgG antibody binds human IL-1B with a binding affinity of Kd= 0.55 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 3.20E−10M. This antibody was used in combination with 6H3.1A4 (anti-IL-1α) antibody for the generation of anti-human IL-1α/β DVD-Ig molecule that had therapeutic potential (PMID: 20068402).

Product Class:	Purified
Clone ID:	6B12.4F6
Synonyms:	IL1B; IL-1β; 1L1F2; Interleukin-1 beta; IL-1 beta; Catabolin
Amount:	100 μg
Application Codes Clone:	ELISA; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds human Interleukin-1 beta, which is a potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. It promotes Th17 differentiation of T-cells and synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. It also plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Application Notes:	The original mouse IgG antibody binds human IL-1B with a binding affinity of Kd= 0.55 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 3.20E−10M. This antibody was used in combination with 6H3.1A4 (anti-IL-1α) antibody for the generation of anti-human IL-1α/β DVD-Ig molecule that had therapeutic potential (PMID: 20068402).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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