Mfg Part Number
Ab03567-10.3
Vector Labs Ab03567-10.3 Anti-Hepcidin-25 [4C11], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing mice with immunized with a N-terminal hepcidin peptide 'DTHFPIC' conjugated with a five amino acid peptide linker (i.e., GPGPG) to an OVA peptide (amino acids 323-336) sequence, i.e., full-length immunogen DTHFPICGPGPGISQAVHAAHAEINE.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | 4C11 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | HAMP; hHepc; HEPC; LEAP1; LEAP-1; Hepcidin; Liver-expressed antimicrobial peptide 1; hepcidin-22 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds human hepcidin-25 in the N-terminus region. This antibody also cross reacts with mouse and rat hepcidin-2, to a much lesser degree, human hepcidin-22 but not human hepcidin-20. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide stabilized by 4 disulfide bonds that plays a major role in innate immunity and iron homeostasis. It controls the delivery of iron to blood plasma from intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and from iron-storing hepatocytes. Hepcidin acts by binding to and inactivating the sole cellular iron exporter, ferroprotein, which delivers iron to plasma from all iron-transporting cells. |
| Application Notes: | This antibody can be used for the detection of human, rat or mouse hepcidin-25 using ELISA. This antibody can also detect human hepcidin-22 with a binding affinity of Kd= 209nM (US8609817). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |