Vector Labs Ab03568-1.1 Anti-Hepcidin-25 [AN-LP1], Mouse IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: AN-LP1
Synonyms: HAMP; hHepc; HEPC; LEAP1; LEAP-1; Hepcidin; Liver-expressed antimicrobial peptide 1; CNCM I-3794
Amount: 100 μg
Application Codes Clone: ELISA; WB; IHC; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for amino acids 'DTHFPICIFCCGCCHRSKCGMCCKT' of human hepcidin-25 and does not cross react with murine hepcidin-25. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide stabilized by 4 disulfide bonds that plays a major role in innate immunity and iron homeostasis. It controls the delivery of iron to blood plasma from intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and from iron-storing hepatocytes. Hepcidin acts by binding to and inactivating the sole cellular iron exporter, ferroprotein, which delivers iron to plasma from all iron-transporting cells.
Application Notes: The original mouse IgG1 antibody binds human hepcidin-25 with a binding affinity of Kd= 99 pM. The binding characterization of this antibody to synthetic human hepcidin-25 was done using ELISA. This antibody can be used for the identification of synthetic human hepcidin or human hepcidin from the sera of patients with an inflammatory disease using dot blot and western blot. This antibody was also used for the immunohistochemical analysis of paraffine-embedded human liver biopsies. The biological activity of this antibody was tested by incubating this antibody with macrophages from J774 murine cell line, and it was reported that this antibody induces ferroprotein degradation (US8487081).

Product Class:	Purified
Clone ID:	AN-LP1
Synonyms:	HAMP; hHepc; HEPC; LEAP1; LEAP-1; Hepcidin; Liver-expressed antimicrobial peptide 1; CNCM I-3794
Amount:	100 μg
Application Codes Clone:	ELISA; WB; IHC; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for amino acids 'DTHFPICIFCCGCCHRSKCGMCCKT' of human hepcidin-25 and does not cross react with murine hepcidin-25. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide stabilized by 4 disulfide bonds that plays a major role in innate immunity and iron homeostasis. It controls the delivery of iron to blood plasma from intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and from iron-storing hepatocytes. Hepcidin acts by binding to and inactivating the sole cellular iron exporter, ferroprotein, which delivers iron to plasma from all iron-transporting cells.
Application Notes:	The original mouse IgG1 antibody binds human hepcidin-25 with a binding affinity of Kd= 99 pM. The binding characterization of this antibody to synthetic human hepcidin-25 was done using ELISA. This antibody can be used for the identification of synthetic human hepcidin or human hepcidin from the sera of patients with an inflammatory disease using dot blot and western blot. This antibody was also used for the immunohistochemical analysis of paraffine-embedded human liver biopsies. The biological activity of this antibody was tested by incubating this antibody with macrophages from J774 murine cell line, and it was reported that this antibody induces ferroprotein degradation (US8487081).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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