Vector Labs Ab03570-3.0 Anti-Hepcidin-25 [19D12], Mouse IgG2b, lambda (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 19D12
Synonyms: HAMP; hHepc; HEPC; LEAP1; LEAP-1; Hepcidin; Liver-expressed antimicrobial peptide 1
Amount: 100 μg
Application Codes Clone: ELISA; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG2 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for amino acids 'DTHFPICIFCCGCCHRSKCGMCCKT' of human hepcidin-25 and does not cross react with murine hepcidin-25. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide stabilized by 4 disulfide bonds that plays a major role in innate immunity and iron homeostasis. It controls the delivery of iron to blood plasma from intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and from iron-storing hepatocytes. Hepcidin acts by binding to and inactivating the sole cellular iron exporter, ferroprotein, which delivers iron to plasma from all iron-transporting cells.
Application Notes: The original IgG antibody binds human hepcidin-25 with a binding affinity of Kd= 340 pM (PMID: 23945155). This antibody can be used in combination with another anti-hepcidin antibody 19D12 for detection of human hepcidin in human serum using a sandwich ELISA. 19D12 was used as a capture antibody and 23F11 was used for detection. The limit of sensitivity for the assay was 30 pg/mL (PMID: 23945155). This antibody was also used for immunohistochemical analysis of formalin-fixed, paraffin-embedded sections of prostrate cancer tissues (PMID: 25858146). This antibody was also used to block hepcidin in a study to confirm the functionality of hepcidin in breast tumor spheroids. As expected, blockade of hepcidin increased ferroprotein, indicating that hepcidin synthesized by these cells functions in an autocrine fashion and exerts its expected biological activity (PMID: 29695834).

Product Class:	Purified
Clone ID:	19D12
Synonyms:	HAMP; hHepc; HEPC; LEAP1; LEAP-1; Hepcidin; Liver-expressed antimicrobial peptide 1
Amount:	100 μg
Application Codes Clone:	ELISA; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG2 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for amino acids 'DTHFPICIFCCGCCHRSKCGMCCKT' of human hepcidin-25 and does not cross react with murine hepcidin-25. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide stabilized by 4 disulfide bonds that plays a major role in innate immunity and iron homeostasis. It controls the delivery of iron to blood plasma from intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and from iron-storing hepatocytes. Hepcidin acts by binding to and inactivating the sole cellular iron exporter, ferroprotein, which delivers iron to plasma from all iron-transporting cells.
Application Notes:	The original IgG antibody binds human hepcidin-25 with a binding affinity of Kd= 340 pM (PMID: 23945155). This antibody can be used in combination with another anti-hepcidin antibody 19D12 for detection of human hepcidin in human serum using a sandwich ELISA. 19D12 was used as a capture antibody and 23F11 was used for detection. The limit of sensitivity for the assay was 30 pg/mL (PMID: 23945155). This antibody was also used for immunohistochemical analysis of formalin-fixed, paraffin-embedded sections of prostrate cancer tissues (PMID: 25858146). This antibody was also used to block hepcidin in a study to confirm the functionality of hepcidin in breast tumor spheroids. As expected, blockade of hepcidin increased ferroprotein, indicating that hepcidin synthesized by these cells functions in an autocrine fashion and exerts its expected biological activity (PMID: 29695834).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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