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Mfg Part Number

Ab03578-30.11

Vector Labs Ab03578-30.11 Anti-Bordetella pertussis cells [scFv 5], scFv fragment (His), ScFv, (50 μg)

VECTOR LABS

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Quick Overview

This antibody was generated by immunizing a BALB/c mouse with 0.1 ml of the commercial pertussis vaccine Quandracel. From this DNA a phage display library was created and panned against B. pertussis whole cells so find this clone.

$692.90

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Description

Product Class: Purified
Clone ID: scFv 5
Heavy Chain Modification: ScFv
Synonyms: filamentous hemagglutinin; FHA; pertactin; PRN; pertactin autotransporter; P.93; Pertactin; Filamentous hemagglutinin transporter protein FhaC; TpsB transporter; fhaC
Amount: 50 μg
Application Codes Clone: ELISA; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for pertactin and filamentous hemagglutinin of Bordetella pertussis. Filamentous hemagglutinin is a member of a two partner secretion pathway (TPS) in which it mediates the secretion of filamentous hemagglutinin FHA (fhaB). Pertactin may have a role in bacterial adhesion, and thus play a role in virulence.
Application Notes: The scFv version of this antibody was used for an ELISA on filamentous hemagglutinin (FHA), pertactin (PRN) and B. pertussis cells. The antibody was positive very every antigen tested. The scFv version of this antibody was tested for its ability to inhibit B. pertussis binding to HEp-2 cells, normally only 35% of cells do not bind to HEp-2 cells but this antibody increases that rate to 75%-100%. Furthermore, the ability of the scFv version of this antibody to prevent B. pertussis infection in mice. The viral load present in mice after 48h was a lot lower than in normal circumstances (Hussein et al, 2007; pmid:17724067).

Product Class:	Purified
Clone ID:	scFv 5
Heavy Chain Modification:	ScFv
Synonyms:	filamentous hemagglutinin; FHA; pertactin; PRN; pertactin autotransporter; P.93; Pertactin; Filamentous hemagglutinin transporter protein FhaC; TpsB transporter; fhaC
Amount:	50 μg
Application Codes Clone:	ELISA; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for pertactin and filamentous hemagglutinin of Bordetella pertussis. Filamentous hemagglutinin is a member of a two partner secretion pathway (TPS) in which it mediates the secretion of filamentous hemagglutinin FHA (fhaB). Pertactin may have a role in bacterial adhesion, and thus play a role in virulence.
Application Notes:	The scFv version of this antibody was used for an ELISA on filamentous hemagglutinin (FHA), pertactin (PRN) and B. pertussis cells. The antibody was positive very every antigen tested. The scFv version of this antibody was tested for its ability to inhibit B. pertussis binding to HEp-2 cells, normally only 35% of cells do not bind to HEp-2 cells but this antibody increases that rate to 75%-100%. Furthermore, the ability of the scFv version of this antibody to prevent B. pertussis infection in mice. The viral load present in mice after 48h was a lot lower than in normal circumstances (Hussein et al, 2007; pmid:17724067).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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