Mfg Part Number
Ab03582-23.0
Vector Labs Ab03582-23.0 Anti-2.4[2.8]2.4-linked Kdo tetrasaccharide [S73-2], Rabbit IgG, kappa (100 μg)
Quick Overview
This antibody was raised by immunizing mice with Kdo(2f8)[Kdo(2f4)]Kdo(2f4)Kdo(2f6)GlcNAc4P-(1f6)GlcNAcol-BSA and further immunizing them with Kdo(2f8)[Kdo(2f4)]Kdo-BSA.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | S73-2 |
| Synonyms: | Kdo; alpha-Kdo; 3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid; 3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid; Chlamydia lipopolysaccharide (LPS) component |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; IF; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for 2.4[2.8]2.4-linked Kdo tetrasaccharide. Which is a partial structure of chlamydial lipopolysaccharide (LPS). |
| Application Notes: | To screen hybridomas for this antibody ELISA was performed on neoglycoconjugates and immunofluorescence was performed on cells infected with either C. trachomatis or C. psittaci. Surface plasmon resonance was performed on Kdo(2f8)Kdo(2f4)KdoBSA (164, 1650, and 2460 RUs), Kdo(2f4)Kdo(2f4)Kdo-BSA, Kdo(2f4)Kdo BSA and BSA(Sigma), as a reference protein using the mouse version of this antibody (Brooks et al, 2010; pmid:20000757). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |