Mfg Part Number
Ab03591-23.0
Vector Labs Ab03591-23.0 Anti-Mycobacterium avium cell lysate [SurfS2.2], Rabbit IgG, lambda (100 μg)
Quick Overview
This antibody was generated by panning a phage library generated from sheep were infected via the oral route with two doses of 109 CFU of a low-passage M. avium subsp. paratuberculosis strain W. against mycobacterial cell suspensions were harvested from culture.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | SurfS2.2 |
| Amount: | 100 μg |
| Application Codes Clone: | WB; FC; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for Mycobacterium avium. Mycobacterium avium together with Mycobacterium intracellulare causes the disease called Mycobacterium avium-intracellulare infection or Mycobacterium avium complex infection in humans. |
| Application Notes: | The scFv version of this antibody was immunoblotted on M. avium subsp. paratuberculosis lysate. The result showed a positive reaction. Furthermore, flow cytometry on cobacterium sp. surface antigen was performed using this antibody, the antibody showed binding to M. avium subsp. avium cells. To determine whether surface-bound SurfS1.2 or SurfS2.2 could influence mycobacterial growth, M. avium subsp. avium was grown in Middlebrook broth, the antibody prevented clumping in M. avium subsp. avium cells. The antibody was also used to magnetically separate M. avium subsp. paratuberculosis from faecal samples (Berger et al, 2006; pmid:16960114). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |