Mfg Part Number
Ab03593-1.1
Vector Labs Ab03593-1.1 Anti-Alpha-crystallin [B4m3], Mouse IgG1, lambda (100 μg)
Quick Overview
This antibody was generated by first panning the Nkuku® library [26] against 20 μg/ml of the M. tuberculosis 16 kDa antigen. Second a sublibrary was created using error-prone PCR. Finally the mutant library was panned against 16 kDa M. tuberculosis antigen.
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| Product Class: | Purified |
|---|---|
| Clone ID: | B4m3 |
| Synonyms: | Acr; hspX; 14 kDa antigen; 16 kDa antigen; Antigen 16kDa (HspX); HSP 16.3; Nox16 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the HspX antigen from Mycobacterium tuberculosis. This antigen acts as a chaperone, as it has a significant ability to suppress the thermal denaturation of alcohol dehydrogenase. Mycobacterium tuberculosis causes the disease tuberculosis. |
| Application Notes: | The binding of the scFv version of this antibody to the antigen was characterised by ELISA. Furthermore, surface plasmon resonance was used to determine the binding kinetics of this antibody on 16 kDa protein of M. tuberculosis (Sixholo et al, 2011; pmid:21349739). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |