Mfg Part Number
Ab03610-21.0
Vector Labs Ab03610-21.0 Anti-Dengue virus envelope protein [C9], Mouse IgM, kappa (50 μg)
Quick Overview
A chimeric library was created from clones that were able to bind DENV2 EDIII, this library was then panned against DENV2 EDIII to create this antibody.
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| Product Class: | Purified |
|---|---|
| Clone ID: | C9 |
| Synonyms: | envelope protein; envelope protein domain 3; EDIII |
| Amount: | 50 μg |
| Application Codes Clone: | ELISA; neutralization; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the EDIII protein of the dengue virus. The dengue virus causes the disease known as dengue fever. |
| Application Notes: | To determine the specificity and DENV cross-reactivity of the IgG1 version of this antibody an ELISA was performed on EDIII, this antibody had strong binding for EDIII from DENV2 and to a lesser extent with EDIII from DENV1 and DENV3. Furthermore, immunofluorescence was performed on uninfected BHK-21 cells and cells infected with DENV2 using the IgG1 version of this antibody, it showed clear fluorescence in the cytoplasm of infected cells. Finally, a plaque reduction neutralization test was performed using the IgG1 version of this antibody, this antibody showed a 25% reduction of plaque formation. This antibody is a chimeric antibody with mouse Vκ/human Cκ and mouse VH/human CH segments (Moreland et al, 2012; pmid:22489114). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |