Vector Labs Ab03613-13.12 Anti-Domain II of the DENV-2 envelope protein (E) [1A5], Human IgG4-S228P, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 1A5
Synonyms: Envelope protein E
Amount: 100 μg
Application Codes Clone: ELISA; IP; WB; neutralization
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Rhesus Macaque Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for domain II of DENV-2 envelope protein. The dengue virus causes the disease known as dengue fever.
Application Notes: To determine the ability of this antibody to bind to different dengue viruses an ELISA was performed. The ELISA was performed using the chimpanzee fab version of this antibody on DENV-1, DENV-2, DENV-3 and DENV-4, it bound well to all of them. Furthermore the specificity of this antibody was determined using radioimmunoprecipitation on the DENV 1 through 4, it showed binding to the envelope protein in all virus variants. The ability of this antibody to bind other flaviviruses was also determined using western blot, the strains used are DENV-1 strain Hawaii; D2, DENV-2 strain New Guinea B; D3, DENV-3 strain H87; D4, DENV-4 strain 814669; WN/D4, WNV/DENV-4 chimera; JE, JEV strain SA14-14-2; LGT, LGTV strain TP 21. It showed only little reaction with JE, JEV strain SA14-14-2; LGT, and LGTV strain TP 21 and a strong reaction with DENV-1 strain Hawaii; D2, DENV-2 strain New Guinea B; D3, DENV-3 strain H87; D4, DENV-4 strain 814669; WN/D4 and WNV/DENV-4 chimera. Finally, a neutralization assay was performed on DENV-1 strain Hawaii; D2, DENV-2 strain New Guinea B; D3, DENV-3 strain H87; D4, DENV-4 strain 814669; WN/D4, WNV/DENV-4 chimera; JE, JEV strain SA14-14-2; LGT, LGTV strain TP 21 using a humanized IgG1 version of this antibody. The antibody was able to neutralize all DENV strains at higher concentrations (Goncalvez et al, 2004; pmid:15542643). While determining the epitope of this antibody a neutralization assay was performed on DENV-2 and DENV-2/DENV-4 chimeras, substitution of Gly106Val or His317Gln in DENV-2 E caused the antibody to no longer be able to neutralize the virus. To semi quantify the affinity of the fab version of this antibody to DENV-2 NGB and its two variants, an ELISA was performed on DENV-2 NGB and its two variants, the second variant showed a strong reduction in binding (Goncalvez et al, 2004; pmid:15542644). An DENV-4 enhancement assay was performed on rhesus monkey primary monocytes using the humanized version of this antibody, at sub neutralizing concentrations the antibody was able to increase the viral load (Goncalvez et al, 2007; pmid:17517625).

Product Class:	Purified
Clone ID:	1A5
Synonyms:	Envelope protein E
Amount:	100 μg
Application Codes Clone:	ELISA; IP; WB; neutralization
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Rhesus Macaque Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for domain II of DENV-2 envelope protein. The dengue virus causes the disease known as dengue fever.
Application Notes:	To determine the ability of this antibody to bind to different dengue viruses an ELISA was performed. The ELISA was performed using the chimpanzee fab version of this antibody on DENV-1, DENV-2, DENV-3 and DENV-4, it bound well to all of them. Furthermore the specificity of this antibody was determined using radioimmunoprecipitation on the DENV 1 through 4, it showed binding to the envelope protein in all virus variants. The ability of this antibody to bind other flaviviruses was also determined using western blot, the strains used are DENV-1 strain Hawaii; D2, DENV-2 strain New Guinea B; D3, DENV-3 strain H87; D4, DENV-4 strain 814669; WN/D4, WNV/DENV-4 chimera; JE, JEV strain SA14-14-2; LGT, LGTV strain TP 21. It showed only little reaction with JE, JEV strain SA14-14-2; LGT, and LGTV strain TP 21 and a strong reaction with DENV-1 strain Hawaii; D2, DENV-2 strain New Guinea B; D3, DENV-3 strain H87; D4, DENV-4 strain 814669; WN/D4 and WNV/DENV-4 chimera. Finally, a neutralization assay was performed on DENV-1 strain Hawaii; D2, DENV-2 strain New Guinea B; D3, DENV-3 strain H87; D4, DENV-4 strain 814669; WN/D4, WNV/DENV-4 chimera; JE, JEV strain SA14-14-2; LGT, LGTV strain TP 21 using a humanized IgG1 version of this antibody. The antibody was able to neutralize all DENV strains at higher concentrations (Goncalvez et al, 2004; pmid:15542643). While determining the epitope of this antibody a neutralization assay was performed on DENV-2 and DENV-2/DENV-4 chimeras, substitution of Gly106Val or His317Gln in DENV-2 E caused the antibody to no longer be able to neutralize the virus. To semi quantify the affinity of the fab version of this antibody to DENV-2 NGB and its two variants, an ELISA was performed on DENV-2 NGB and its two variants, the second variant showed a strong reduction in binding (Goncalvez et al, 2004; pmid:15542644). An DENV-4 enhancement assay was performed on rhesus monkey primary monocytes using the humanized version of this antibody, at sub neutralizing concentrations the antibody was able to increase the viral load (Goncalvez et al, 2007; pmid:17517625).

Specifications

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Brand	Vector Labs
UNSPSC	12161500

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