Mfg Part Number
Ab03618-1.159
Vector Labs Ab03618-1.159 Anti-GFP [Minimizer], Mouse IgG1-Fc Fusion, (100 μg)
Quick Overview
The original antibody was generated by immunizing camels with GFP. A phage display library was constructed and the antibody was selected by panning against GFP.
$692.90
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| Product Class: | Purified |
|---|---|
| Clone ID: | Minimizer |
| Synonyms: | Green fluorescent protein; MI; GBP4 |
| Amount: | 100 μg |
| Application Codes Clone: | Crystallization; in vitro |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for green fluorescent protein. The antibody interacts with β-strands 6 and 7 of GFP. |
| Application Notes: | The antibody could reversibly reduce GFP fluorescence by a factor of 5 in vitro. A comparable fluorescence modulation was also observed after addition of the antibody to soluble cell extract derived from human embryonic kidney (HEK) 293T cells expressing eGFP. The crystal structure of the GFP–antibody complex was determined. The dissociation constant of the antibody was measured (Kd= 0.45 nM) (Kirchhofer et al., 2010; PMID: 20010839). Fluorescence modulation in living cells was also observed after the addition of the antibody to HeLa cells expressing GFP (Schmidthals et al., 2010; PMID: 20372881). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |