Mfg Part Number
Ab03618-1.159-BS
Vector Labs Ab03618-1.159-BS Anti-GFP [Minimizer], Mouse IgG1-Fc Fusion, (1 mg)
Quick Overview
The original antibody was generated by immunizing camels with GFP. A phage display library was constructed and the antibody was selected by panning against GFP.
$5,162.30
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | Minimizer |
| Synonyms: | Green fluorescent protein; MI; GBP4 |
| Amount: | 1 mg |
| Application Codes Clone: | Crystallization; in vitro |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS only. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for green fluorescent protein. The antibody interacts with β-strands 6 and 7 of GFP. |
| Application Notes: | The antibody could reversibly reduce GFP fluorescence by a factor of 5 in vitro. A comparable fluorescence modulation was also observed after addition of the antibody to soluble cell extract derived from human embryonic kidney (HEK) 293T cells expressing eGFP. The crystal structure of the GFP–antibody complex was determined. The dissociation constant of the antibody was measured (Kd= 0.45 nM) (Kirchhofer et al., 2010; PMID: 20010839). Fluorescence modulation in living cells was also observed after the addition of the antibody to HeLa cells expressing GFP (Schmidthals et al., 2010; PMID: 20372881). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |