Vector Labs Ab03626-10.29 Anti-HA [Mab 3.1], Human Fab fragment, His-Tagged, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: Mab 3.1
Heavy Chain Modification: His-Tagged
Synonyms: Hemagglutinin
Amount: 100 μg
Application Codes Clone: ELISA; neutralization; in vivo; crystallization
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: The antibody recognizes the conserved heterosubtypic epitope in the stem of influenza A virus hemagglutinin. The epitope consists of residues from the N- and C-terminal regions of HA1 (38, 40 to 42, 289 to 293, and 318) and the N-terminal portion of HA2 (18 to 21, 38, 41, 42, 45, 49, 52, 53, 56), including helix A. The antibody neutralizes influenza viruses from the H1a clade but has little neutralizing activity against the H1b clade. The antibody binds HA proteins from A/Puerto Rico/8/1934(H1N1), and A/Japan/305/1957(H2N2), A/flat-faced bat/Peru/033/2010(H18N1) virus, A/duck/Alberta/345/1976(H1N1), A/USSR/90/1977(H1N1), A/Beijing/262/1995(H1N1), A/Solomon Islands/3/2006(H1N1), A/Adachi/2/1957(H2N2), A/Vietnam/1203/2004(H5N1), and A/turkey/Massachusetts/3740/1965(H6N2) viruses. It fails to bind to A/Victoria/3/1975(H3N2) and A/fowlplague/Bratislava/1979(H7N7), A/little yellow-shouldered bat/Guatemala/164/2009(H17N10) virus,A/duck/Alberta/60/1976(H12N5), A/gull/Maryland/704/1977(H13N6), A/black-headed gull/Sweden/4/1999(H16N3), A/duck/Ukraine/1/1963(H3N8), A/Hong Kong/1/1968(H3N2), A/duck/Czechoslovakia/1956(H4N6), A/Netherlands/219/2003(H7N7), A/chicken/Germany/N/1949(H10N7), A/mallard/Astrakhan/263/1982(H14N5), or A/shearwater/W. Australia/2576/1979(H15N9) viruses.
Application Notes: In ELISAs, the Fab fragment was found to bind to recombinant HA protein. The Fab fragment neutralized viruses of the H1, H2, H5, and H6 subtypes at half-maximal inhibitory concentrations in the ug/ml range. Passive immunization with the Fab fragment protected mice from a lethal challenge with A/Puerto Rico/8/1934(H1N1) virus. The crystal structure of the antibody in complex with HA from a pandemic H1N1 influenza virus was determined.

Product Class:	Purified
Clone ID:	Mab 3.1
Heavy Chain Modification:	His-Tagged
Synonyms:	Hemagglutinin
Amount:	100 μg
Application Codes Clone:	ELISA; neutralization; in vivo; crystallization
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	The antibody recognizes the conserved heterosubtypic epitope in the stem of influenza A virus hemagglutinin. The epitope consists of residues from the N- and C-terminal regions of HA1 (38, 40 to 42, 289 to 293, and 318) and the N-terminal portion of HA2 (18 to 21, 38, 41, 42, 45, 49, 52, 53, 56), including helix A. The antibody neutralizes influenza viruses from the H1a clade but has little neutralizing activity against the H1b clade. The antibody binds HA proteins from A/Puerto Rico/8/1934(H1N1), and A/Japan/305/1957(H2N2), A/flat-faced bat/Peru/033/2010(H18N1) virus, A/duck/Alberta/345/1976(H1N1), A/USSR/90/1977(H1N1), A/Beijing/262/1995(H1N1), A/Solomon Islands/3/2006(H1N1), A/Adachi/2/1957(H2N2), A/Vietnam/1203/2004(H5N1), and A/turkey/Massachusetts/3740/1965(H6N2) viruses. It fails to bind to A/Victoria/3/1975(H3N2) and A/fowlplague/Bratislava/1979(H7N7), A/little yellow-shouldered bat/Guatemala/164/2009(H17N10) virus,A/duck/Alberta/60/1976(H12N5), A/gull/Maryland/704/1977(H13N6), A/black-headed gull/Sweden/4/1999(H16N3), A/duck/Ukraine/1/1963(H3N8), A/Hong Kong/1/1968(H3N2), A/duck/Czechoslovakia/1956(H4N6), A/Netherlands/219/2003(H7N7), A/chicken/Germany/N/1949(H10N7), A/mallard/Astrakhan/263/1982(H14N5), or A/shearwater/W. Australia/2576/1979(H15N9) viruses.
Application Notes:	In ELISAs, the Fab fragment was found to bind to recombinant HA protein. The Fab fragment neutralized viruses of the H1, H2, H5, and H6 subtypes at half-maximal inhibitory concentrations in the ug/ml range. Passive immunization with the Fab fragment protected mice from a lethal challenge with A/Puerto Rico/8/1934(H1N1) virus. The crystal structure of the antibody in complex with HA from a pandemic H1N1 influenza virus was determined.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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