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Mfg Part Number

Ab03659-3.0

Vector Labs Ab03659-3.0 Anti-Citrus tristeza virus coat protein [3DF1], Mouse IgG2b, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing BALB/c mice intraperitoneally with Citrus tristeza virus (isolate T-308) preparation.

$570.70

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Description

Product Class: Purified
Clone ID: 3DF1
Synonyms: CP; CTV; CTV capsid protein; CTV coat protein; capsid protein, coat protein; p25 major coat protein
Amount: 100 μg
Application Codes Clone: ELISA; neutralize
Shipping Temperature: Wet Ice
Origin Pub PMID: 18943374
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds the p25 major coat protein of the Citrus tristeza virus (CTV). This antibody cannot bind the CTV isolate T-397-P. Tristeza is the most important virus disease of citrus worldwide. It is caused by citrus tristeza virus (CTV), a closterovirus that is naturally spread in the fields by aphids in a semipersistent manner and is also easily transmitted by grafting. CTV is a monopartite, single stranded RNA virus encapsulated in a coat protein.
Application Notes: This antibody can be used for the detection of Citrus tristeza virus in crude extracts of plants infected with isolates T-300 or T-308 using ELISA and double antibody sandwich ELISA. This antibody reacted with 20 CTV isolates from different Spanish citrus areas as well as with CTV isolates from California (T-514) and two unclassified from Iran and Vietnam in an ELISA (Vela et al., 1986). Transgenic plants expressing this antibody showed both a delay in symptom appearance and attenuation of symptom intensity. Overall, 40–60% of the plants were resistant, as revealed by the absence of virus antigens in tissue-print ELISA (PMID: 20204695).

Product Class:	Purified
Clone ID:	3DF1
Synonyms:	CP; CTV; CTV capsid protein; CTV coat protein; capsid protein, coat protein; p25 major coat protein
Amount:	100 μg
Application Codes Clone:	ELISA; neutralize
Shipping Temperature:	Wet Ice
Origin Pub PMID:	18943374
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds the p25 major coat protein of the Citrus tristeza virus (CTV). This antibody cannot bind the CTV isolate T-397-P. Tristeza is the most important virus disease of citrus worldwide. It is caused by citrus tristeza virus (CTV), a closterovirus that is naturally spread in the fields by aphids in a semipersistent manner and is also easily transmitted by grafting. CTV is a monopartite, single stranded RNA virus encapsulated in a coat protein.
Application Notes:	This antibody can be used for the detection of Citrus tristeza virus in crude extracts of plants infected with isolates T-300 or T-308 using ELISA and double antibody sandwich ELISA. This antibody reacted with 20 CTV isolates from different Spanish citrus areas as well as with CTV isolates from California (T-514) and two unclassified from Iran and Vietnam in an ELISA (Vela et al., 1986). Transgenic plants expressing this antibody showed both a delay in symptom appearance and attenuation of symptom intensity. Overall, 40–60% of the plants were resistant, as revealed by the absence of virus antigens in tissue-print ELISA (PMID: 20204695).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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