Mfg Part Number
Ab03660-3.0
Vector Labs Ab03660-3.0 Anti-Citrus tristeza virus coat protein [3CA5], Mouse IgG2b, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice intraperitoneally with Citrus tristeza virus (isolate T-308) preparation.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 3CA5 |
| Synonyms: | CP; CTV; CTV capsid protein; CTV coat protein; capsid protein, coat protein; p25 major coat protein |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; neutralize |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 18943374 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds the p25 major coat protein of the Citrus tristeza virus (CTV). Tristeza is the most important virus disease of citrus worldwide. It is caused by citrus tristeza virus (CTV), a closterovirus that is naturally spread in the fields by aphids in a semipersistent manner and is also easily transmitted by grafting. CTV is a monopartite, single stranded RNA virus encapsulated in a coat protein. |
| Application Notes: | This antibody can be used for the detection of Citrus tristeza virus in crude extracts of plants infected with isolates T-300 or T-308 using ELISA and double antibody sandwich ELISA. This antibody reacted with 20 CTV isolates from different Spanish citrus areas as well as with CTV isolates from California (T-514) and two unclassified from Iran and Vietnam in an ELISA (Vela et al., 1986). Transgenic plants expressing this antibody showed both a delay in symptom appearance and attenuation of symptom intensity. Overall, 40–60% of the plants were resistant, as revealed by the absence of virus antigens in tissue-print ELISA (PMID: 20204695). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |