Vector Labs Ab03666-23.0 Anti-Potato virus Y NIa [22-19], Rabbit IgG, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 22-19
Synonyms: PVY; PVY NIa; NIa; Potato virus Y; Genome polyprotein; Nuclear inclusion a; aphid-borne virus; 22-1; Hybridoma 22
Amount: 100 μg
Application Codes Clone: ELISA; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody bind the nuclear inclusion A (NIa) protein of the Potato virus Y (PVY). Potato virus Y is a monopartite, single stranded RNA virus, plant pathogen that infects mainly Solanaceous plants including, potato, tomato, pepper, tobacco and eggplant. There are multiple strains of the virus including the common strain, PVYO, which causes mosaic symptoms in most hosts. The necrotic strains PVYN and PVYNTN both cause a veinal necrosis on tobacco; PVYNTN also causes a tuber necrosis in certain potato varieties. This necrosis is referred to as potato tubers necrotic ringspot disease (PTNRD). In general, the necrotic strains tend to cause a more mild mosaic symptom than PVYO in potato. PVY is transmitted in a non-persistent manner by aphids.
Application Notes: This antibody was used for in situ localization of potato virus Y NIa protein using immunofluorescence staining. The staining showed the accumulation of the NIa protein in both nucleus and cytoplasm. The binding characterization of the antibody was done using ELISA (PMID: 16884787). In vivo, this antibody was able to detect different forms of the NIa protein including precursors and cleavage products and also able to inhibit the cleavage of the polyprotein detected in the semipurified NI (PMID: 11556707).

Product Class:	Purified
Clone ID:	22-19
Synonyms:	PVY; PVY NIa; NIa; Potato virus Y; Genome polyprotein; Nuclear inclusion a; aphid-borne virus; 22-1; Hybridoma 22
Amount:	100 μg
Application Codes Clone:	ELISA; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody bind the nuclear inclusion A (NIa) protein of the Potato virus Y (PVY). Potato virus Y is a monopartite, single stranded RNA virus, plant pathogen that infects mainly Solanaceous plants including, potato, tomato, pepper, tobacco and eggplant. There are multiple strains of the virus including the common strain, PVYO, which causes mosaic symptoms in most hosts. The necrotic strains PVYN and PVYNTN both cause a veinal necrosis on tobacco; PVYNTN also causes a tuber necrosis in certain potato varieties. This necrosis is referred to as potato tubers necrotic ringspot disease (PTNRD). In general, the necrotic strains tend to cause a more mild mosaic symptom than PVYO in potato. PVY is transmitted in a non-persistent manner by aphids.
Application Notes:	This antibody was used for in situ localization of potato virus Y NIa protein using immunofluorescence staining. The staining showed the accumulation of the NIa protein in both nucleus and cytoplasm. The binding characterization of the antibody was done using ELISA (PMID: 16884787). In vivo, this antibody was able to detect different forms of the NIa protein including precursors and cleavage products and also able to inhibit the cleavage of the polyprotein detected in the semipurified NI (PMID: 11556707).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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