Vector Labs Ab03667-3.0 Anti-Tobacco mosaic virus [Mab24], Mouse IgG2b, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: Mab24
Synonyms: TMV; neotope; intact TMV virions; intact birus; Nicotiana tabacum; rAb24
Amount: 100 μg
Application Codes Clone: ELISA; neutralize
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody recognizes a neotope on the surface of intact Tobacco mosaic virus (TMV) particles. TMV is a positive-sense single-stranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially tobacco. Symptoms induced by Tobacco mosaic virus (TMV) are somewhat dependent on the host plant and can include mosaic, mottling, necrosis, stunting, leaf curling, and yellowing of plant tissues.
Application Notes: Infection of transgenic F1 plants producing mAb24-P with TMV showed a reduction of necrotic lesion numbers which is correlated with the amount of antibody produced in transgenic plants. This antibody is capable of inhibiting the infectivity of the virus if TMV is adsorbed in vitro at ratios as low as 300 antibody molecules per virion prior to inoculation (Voss et al., 1995). This antibody was used in the generation of a bispecific antibody biscFv2429 in transgenic suspension culture cells and tobacco plants, wherein two antibodies scFv24 and scFv29 were connected by the Trichoderma reesi cellobiohydrolase I linker. These antibodies bind two different epitopes of TMV. The epitope specificity of this antibody was determined using ELISA (PMID: 10411643). This antibody was used for the magnetic immunodetection of TMV (PMID: 25710366). This antibody was also used in the generation of recombinant fusion proteins scFv24-PDGFR and scFv24-TcRβ in transgenic tobacco suspension cultures and transgenic plants. Bioassays of viral infection showed that transgenic tobacco plants expressing scFv24-TcRβ were resistant to TMV infection (Schillberg et al., 2000).

Product Class:	Purified
Clone ID:	Mab24
Synonyms:	TMV; neotope; intact TMV virions; intact birus; Nicotiana tabacum; rAb24
Amount:	100 μg
Application Codes Clone:	ELISA; neutralize
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody recognizes a neotope on the surface of intact Tobacco mosaic virus (TMV) particles. TMV is a positive-sense single-stranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially tobacco. Symptoms induced by Tobacco mosaic virus (TMV) are somewhat dependent on the host plant and can include mosaic, mottling, necrosis, stunting, leaf curling, and yellowing of plant tissues.
Application Notes:	Infection of transgenic F1 plants producing mAb24-P with TMV showed a reduction of necrotic lesion numbers which is correlated with the amount of antibody produced in transgenic plants. This antibody is capable of inhibiting the infectivity of the virus if TMV is adsorbed in vitro at ratios as low as 300 antibody molecules per virion prior to inoculation (Voss et al., 1995). This antibody was used in the generation of a bispecific antibody biscFv2429 in transgenic suspension culture cells and tobacco plants, wherein two antibodies scFv24 and scFv29 were connected by the Trichoderma reesi cellobiohydrolase I linker. These antibodies bind two different epitopes of TMV. The epitope specificity of this antibody was determined using ELISA (PMID: 10411643). This antibody was used for the magnetic immunodetection of TMV (PMID: 25710366). This antibody was also used in the generation of recombinant fusion proteins scFv24-PDGFR and scFv24-TcRβ in transgenic tobacco suspension cultures and transgenic plants. Bioassays of viral infection showed that transgenic tobacco plants expressing scFv24-TcRβ were resistant to TMV infection (Schillberg et al., 2000).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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