Mfg Part Number
Ab03678-23.0
Vector Labs Ab03678-23.0 Anti-Usutu virus envelop protein E [A9], Rabbit IgG, lambda (100 μg)
Quick Overview
A DIII‐immune phage display scFv library was generated by immunizing three chickens against group A and group I USUV DIII. The original antibody was isolated from this library by panning against Usutu virus A and Usutu virus B.
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| Product Class: | Purified |
|---|---|
| Clone ID: | A9 |
| Synonyms: | USUV; USUV genome polyprotein; USUV envelope protein; genome polyprotein; envelope protein E; Usutu virus; West Nile virus; WNV; Domain III (DIII); flavivirus |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; WB |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 32829514 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds an epitope within domain III of the envelope protein of Usutu virus (USUV). Usutu virus is a mosquito-borne flavivirus belonging to the Japanese encephalitis virus (JEV) serocomplex, which is an emerging zoonotic arbovirus of concern because of its pathogenicity to humans. This virus propagates via a zoonotic life cycle with birds as amplifying hosts and ornithophilic mosquitoes acting as vectors. USUV infections cause mild influenza‐like symptoms, in sever cases invasion of the central nervous system in immunocompromised patients causing meningoencephalitis and meningitis. |
| Application Notes: | This antibody can bind the E protein domain III antigens of USUV strains A, B, I and West Nile virus in an ELISA. Surface plasmon resonance was used for the determination of binding affinity of this scFv antibody. The scFv antibody binds USUS I and WNV domain III with a Kd of 0.29–0.38 μM and 0.10 μM respectively. This antibody can also detect USUV E protein DIII from groups A, B, and I as well as WNV 2741 E protein DIII in a western blot (PMID: 32829514). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |