Vector Labs Ab03703-13.0 Anti-ACE2 [h11B11], Human IgG4, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: h11B11
Synonyms: Angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; ACEH; Angiotensin-converting enzyme-related carboxypeptidase; ACE-related carboxypeptidase; Metalloprotease MPROT15; 11B11
Amount: 100 μg
Application Codes Clone: ELISA; FC; neutralize; inhibit
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG4
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds the extracellular domain of the human ACE2 protein and cross reacts with cynomolgus monkey ACE2 protein. Angiotensin-converting enzyme 2 (ACE2) is a carboxypeptidase enzyme which catalyzes the cleavage of angiotensin I to angiotensin 1-9 and angiotensin II into the vasodilator angiotensin 1-7. This enzyme is a transmembrane protein found on the surface of arteries, heart, kidneys, testis and epithelia of the lung and small intestine. The protein acts as a receptor for spike protein of human coronaviruses SARS-CoV and SARS-CoV-2/2019-nCoV (COVID-19), as well as human coronavirus NL63/HCoV-NL63 and plays a role in virus entry into target cells. Some strategies to treat COVID-19 include blockade of ACE2 receptor and delivery of excess soluble form of ACE2.
Application Notes: This antibody can bind purified human ACE2 recombinant protein in an ELISA. The ability of this antibody to bind ACE2 and block its interaction with SARS-CoV-RBD and SARS-CoV-2-RBD was determined using flow cytometry. The binding affinity of the IgG1 version of this antibody to wild type human ACE2 was found to be Kd= 1.95 nM. This antibody was also capable of binding mutated versions of human ACE2 like I21T, K26R, N33D and D38E. This antibody does not inhibit the carboxypeptidase activity of hACE2 in vitro. This antibody showed strong inhibitory activity against SARS-CoV, SARS-CoV-2, and epidemic SARS-CoV-2 variant pseudo virus in vitro. When administered therapeutically or prophylactically in the hACE2 mouse model, this antibody alleviates and prevents SARS-CoV-2 replication and virus-induced pathological syndromes. This antibody also didn't exhibit any significant toxicity to cynomolgus monkey when administered in high doses (PMID: 34404805).

Product Class:	Purified
Clone ID:	h11B11
Synonyms:	Angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; ACEH; Angiotensin-converting enzyme-related carboxypeptidase; ACE-related carboxypeptidase; Metalloprotease MPROT15; 11B11
Amount:	100 μg
Application Codes Clone:	ELISA; FC; neutralize; inhibit
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG4
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds the extracellular domain of the human ACE2 protein and cross reacts with cynomolgus monkey ACE2 protein. Angiotensin-converting enzyme 2 (ACE2) is a carboxypeptidase enzyme which catalyzes the cleavage of angiotensin I to angiotensin 1-9 and angiotensin II into the vasodilator angiotensin 1-7. This enzyme is a transmembrane protein found on the surface of arteries, heart, kidneys, testis and epithelia of the lung and small intestine. The protein acts as a receptor for spike protein of human coronaviruses SARS-CoV and SARS-CoV-2/2019-nCoV (COVID-19), as well as human coronavirus NL63/HCoV-NL63 and plays a role in virus entry into target cells. Some strategies to treat COVID-19 include blockade of ACE2 receptor and delivery of excess soluble form of ACE2.
Application Notes:	This antibody can bind purified human ACE2 recombinant protein in an ELISA. The ability of this antibody to bind ACE2 and block its interaction with SARS-CoV-RBD and SARS-CoV-2-RBD was determined using flow cytometry. The binding affinity of the IgG1 version of this antibody to wild type human ACE2 was found to be Kd= 1.95 nM. This antibody was also capable of binding mutated versions of human ACE2 like I21T, K26R, N33D and D38E. This antibody does not inhibit the carboxypeptidase activity of hACE2 in vitro. This antibody showed strong inhibitory activity against SARS-CoV, SARS-CoV-2, and epidemic SARS-CoV-2 variant pseudo virus in vitro. When administered therapeutically or prophylactically in the hACE2 mouse model, this antibody alleviates and prevents SARS-CoV-2 replication and virus-induced pathological syndromes. This antibody also didn't exhibit any significant toxicity to cynomolgus monkey when administered in high doses (PMID: 34404805).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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